AB179523

Anti-PKC 抗体 [EPR16791]

Name: Anti-PKC 抗体 [EPR16791] (ab179523) | Abcam
Brand: Abcam Limited
SKU: AB179523
Price: 51000 JPY
Availability: InStock
Rating: 3 (1 reviews)

Anti-PKC antibody [EPR16791]

RabMAb
Recombinant
詳細を見る

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal KPCL antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 5 publications.

別名を表示する

Pkch, Prkch, Protein kinase C eta type, PKC-L, nPKC-eta

8 Images

Supplier Data

Immunoprecipitation - Anti-PKC antibody [EPR16791] (AB179523)

PKC was immunoprecipitated from 1 mg of Mouse lung whole cell lysate with ab179523 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179523 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Mouse lung whole cell lysate, 10μg (Input).

Lane 2 : ab179523 IP in Mouse lung whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal[EPR25A]-Isotype Control (ab172730) instead of ab179523 in Mouse lung whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-PKC antibody [EPR16791] (ab179523)

Predicted band size: 77 kDa

false

Supplier Data

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Blocking/Dilution buffer : 5% NFDM/TBST.

The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKCs (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331.

Newton AC, 1995. J. Biol. Chem. 270, 28495-28498).

All lanes:

Western blot - Anti-PKC antibody [EPR16791] (ab179523) at 1/10000 dilution

Lane 1:

Human fetal skin tissue lysate at 10 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 4:

Mouse lung tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 77 kDa

Observed band size: 40 kDa,78 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Blocking/Dilution buffer : 5% NFDM/TBST.

Active human PKC zeta full length protein (Catalog# ab60848) contains aa1-592 with GST-tag; Active human PKC iota full length protein (Catalog#ab60850) contains aa1-596 with GST-tag.

All lanes:

Western blot - Anti-PKC antibody [EPR16791] (ab179523) at 1/1000 dilution

Lane 1:

Active human PKC zeta full length protein at 0.02 µg

Lane 2:

Active human PKC iota full length protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

Supplier Data

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Blocking/Dilution buffer : 5% NFDM/TBST.

Active human PKC alpha full length protein (Catalog#ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (Catalog#ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (Catalog#ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein (Catalog#ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (Catalog#ab60844) contains aa1-676 with GST-tag; Active human PKC mu full length protein (Catalog#ab60873) contains aa1-912 with GST-tag.

All lanes:

Western blot - Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

Lane 1:

Active human PKC alpha full length protein at 0.02 µg

Lane 2:

Active human PKC beta 1 full length protein at 0.02 µg

Lane 3:

Active human PKC beta 2 full length protein at 0.02 µg

Lane 4:

Active human PKC gamma full length protein at 0.02 µg

Lane 5:

Active human PKC delta full length protein at 0.02 µg

Lane 6:

Active human PKC mu full length protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 77 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

All lanes:

Human fetal heart tissue lysate at 1/10 dilution

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 126 kDa,150 kDa,77 kDa

Observed band size: 127 kDa,130 kDa,180 kDa,78 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Blocking/Dilution buffer : 5% NFDM/TBST.

Active human PKC eta full length protein (Catalog#ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (Catalog#ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (Catalog#ab56641) contains aa1-706 with GST-tag.

All lanes:

Western blot - Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

Lane 1:

Active human PKC eta full length protein at 0.02 µg

Lane 2:

Active human PKC epsilon full length protein at 0.02 µg

Lane 3:

Active human PKC theta full length protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 77 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Blocking/Dilution buffer : 5% NFDM/TBST.

The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKCs (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331.

Newton AC, 1995. J. Biol. Chem. 270, 28495-28498).

All lanes:

Western blot - Anti-PKC antibody [EPR16791] (ab179523) at 1/2000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat heart lysate at 10 µg

Lane 5:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 77 kDa

Observed band size: 40 kDa,78 kDa

false

Exposure time: 15s

Unknown

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Recombinant PKC isoforms were tested for reactivity by western blot, and are marked as positive (+) or negative (-).

All lanes:

Western blot - Anti-PKC antibody [EPR16791] (ab179523)

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16791

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" } } }

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製品の詳細

Recombinant PKC isoforms were tested for reactivity to ab179521 by western blot, and are marked as positive (+) or negative (-).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C (PKC) comprises a family of serine/threonine kinases with several isoforms such as PKC alpha beta and gamma among others. PKC enzymes play key roles in intracellular signaling particularly in mediating responses to growth factors hormones and other signals. PKC exhibits a molecular weight generally ranging around 77 to 97 kDa depending on the isoform. These enzymes show expression in many tissues but have high concentrations in the brain heart and lung. PKC activation often involves translocation to cellular membranes which is important for their signaling roles.

Biological function summary

PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.

Pathways

PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli.

PKC is linked to cancer and cardiovascular diseases. In cancer dysregulation of PKC activity often contributes to tumor growth and progression by altering pathways that control cell division and survival. PKC abnormally interacts with proteins like Bcl-2 promoting cell survival. In cardiovascular diseases changes in PKC signaling can impact heart function contributing to conditions like heart failure through interactions with proteins such as troponin. Research on PKC and these diseases can provide insights into developing targeted therapies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

See full target information Prkch

追加のターゲット

PRKCB,PRKCA

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed40280116

2025

Understanding the role of iron/heme metabolism in the anti‑inflammatory effects of natural sulfur molecules against lipopolysaccharide‑induced inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Young Kang,Se Won Bae,Kyoung-Jin Jang

Journal of cellular physiology 239:e31446 PubMed39311471

2024

Maslinic acid prevented lipopolysaccharide-induced injury of IPEC-J2 cells through regulating PTEN-FAK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Hao Yu,Aike Li,Ting Wang,Qiyuan Wang,Huiyu Qi,Chuanqi Wang,Jing Zhang

Life (Basel, Switzerland) 11: PubMed34068523

2021

Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Nipin Sp,Dong Young Kang,Hyoung Do Kim,Alexis Rugamba,Eun Seong Jo,Jong-Chan Park,Se Won Bae,Jin-Moo Lee,Kyoung-Jin Jang

Molecules (Basel, Switzerland) 25: PubMed32429534

2020

Sulfur Compounds Inhibit High Glucose-Induced Inflammation by Regulating NF-κB Signaling in Human Monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Seong Jo,Nipin Sp,Dong Young Kang,Alexis Rugamba,Il Ho Kim,Se Won Bae,Qing Liu,Kyoung-Jin Jang,Young Mok Yang

Medical science monitor : international medical jo 25:4082-4093 PubMed31152645

2019

Acupuncture Administration Improves Cognitive Functions and Alleviates Inflammation and Nuclear Damage by Regulating Phosphatidylinositol 3 Kinase (PI3K)/Phosphoinositol-Dependent Kinase 1 (PDK1)/Novel Protein Kinase C (nPKC)/Rac 1 Signaling Pathway in Senescence-Accelerated Prone 8 (SAM-P8) Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Guomin Li,Lirong Zeng,Haiyan Cheng,Jingxian Han,Xuezhu Zhang,Hui Xie

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKC 抗体 [EPR16791]

Anti-PKC antibody [EPR16791]

Immunoprecipitation - Anti-PKC antibody [EPR16791] (AB179523)

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

Western blot - Anti-PKC antibody [EPR16791] (AB179523)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (5)

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Abcam product promise