Anti-PKC alpha (phospho S657 + Y658) 抗体 (ab23513)
Key features and details
- Rabbit polyclonal to PKC alpha (phospho S657 + Y658)
- Suitable for: ICC, WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-PKC alpha (phospho S657 + Y658) antibody
PKC alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC alpha (phospho S657 + Y658) -
由来種
Rabbit -
特異性
ab23513 detected PKC alpha and PKC beta I, but not other PKC isoforms in rat brain lysate. -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide corresponding to Human PKC alpha (phospho S657 + Y658).
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特記事項
We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23513の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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WB | (4) |
1/1000. Detects a band of approximately 82 kDa.
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特記事項 |
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ICC
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 82 kDa. |
ターゲット情報
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機能
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. Cell membrane. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 24680 Rat
- SwissProt: P05696 Rat
- Unigene: 207908 Rat
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別名
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
see all
画像
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Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658).
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Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKCα (Ser-657/Tyr-658) (PP1091) antibody in the absence (Left) or presence (Right) of blocking peptide (PX1095). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594
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Immunofluorescence analysis of S2-013 cells, staining PKC alpha (phospho S657 + Y658) with ab23513.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with blocking solution (3% BSA/PBS). Cells were incubated with primary antibody for 1 hour. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab23513 は 22 報の論文で使用されています。
- Teegala LR et al. Protein Kinase C α and β compensate for each other to promote stem cell factor-mediated KIT phosphorylation, mast cell viability and proliferation. FASEB J 36:e22273 (2022). PubMed: 35349200
- Jiang Y et al. Aquaporin 1 mediates early responses to osmotic stimuli in endothelial cells via the calmodulin pathway. FEBS Open Bio 11:75-84 (2021). PubMed: 33125833
- Wu A et al. Delphinidin induces cell cycle arrest and apoptosis in HER-2 positive breast cancer cell lines by regulating the NF-κB and MAPK signaling pathways. Oncol Lett 22:832 (2021). PubMed: 34712357
- Komleva YK et al. NLRP3 Inflammasome Blocking as a Potential Treatment of Central Insulin Resistance in Early-Stage Alzheimer's Disease. Int J Mol Sci 22:N/A (2021). PubMed: 34769018
- Lenkavska L et al. Searching for combination therapy by clustering methods: Stimulation of PKC in Golgi apparatus combined with hypericin induced PDT. Photodiagnosis Photodyn Ther N/A:101813 (2020). PubMed: 32442674