Anti-PKA R2/PKR2 抗体 (ab38949)
Key features and details
- Rabbit polyclonal to PKA R2/PKR2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKA R2/PKR2 antibody
PKA R2/PKR2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKA R2/PKR2 -
由来種
Rabbit -
特異性
ab38949 binds to PKARIIa, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIb, PKARIIb) and can recognize native or reduced PKARIIa. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab38949 has been peptide-affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38949の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. -
組織特異性
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. -
配列類似性
Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains. -
翻訳後修飾
Phosphorylated by the activated catalytic chain. -
細胞内局在
Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5576 Human
- Omim: 176910 Human
- SwissProt: P13861 Human
- Unigene: 631923 Human
- Unigene: 731573 Human
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別名
- cAMP dependent protein kinase regulatory subunit alpha 2 antibody
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- cAMP dependent protein kinase type II alpha regulatory chain antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (ab38949)ICC/IF image of ab38949 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (ab38949)ICC/IF image of ab38949 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab38949 は 18 報の論文で使用されています。
- LaCroix R et al. Complex effects of kinase localization revealed by compartment-specific regulation of protein kinase A activity. Elife 11:N/A (2022). PubMed: 35199643
- Xuan W et al. Propofol Protects Against Erastin-Induced Ferroptosis in HT-22 Cells. J Mol Neurosci 72:1797-1808 (2022). PubMed: 35727524
- Zhan Y et al. Maren Pills Improve Constipation via Regulating AQP3 and NF-?B Signaling Pathway in Slow Transit Constipation In Vitro and In Vivo. Evid Based Complement Alternat Med 2020:9837384 (2020). PubMed: 32774435
- Xiao J et al. Protective Effects of Adiponectin against Cobalt Chloride-Induced Apoptosis of Smooth Muscle Cells via cAMP/PKA Pathway. Biomed Res Int 2020:7169348 (2020). PubMed: 33102590
- Zhang F et al. Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIa activity. Exp Ther Med 17:748-758 (2019). PubMed: 30651859