Anti-PKA beta (catalytic subunit) 抗体 (ab187515)
Key features and details
- Rabbit polyclonal to PKA beta (catalytic subunit)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKA beta (catalytic subunit) antibody
PKA beta (catalytic subunit) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKA beta (catalytic subunit) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Pig
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免疫原
Recombinant full length protein corresponding to Human PKA beta (catalytic subunit) aa 1 to the C-terminus.
Database link: P22694 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187515の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 41 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. Predicted molecular weight: 41 kDa. |
ターゲット情報
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機能
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. -
組織特異性
Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. -
細胞内局在
Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5567 Human
- Omim: 176892 Human
- SwissProt: P05131 Cow
- SwissProt: P22694 Human
- SwissProt: P05383 Pig
- Unigene: 487325 Human
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別名
- cAMP-dependent protein kinase catalytic beta subunit isoform 4ab antibody
- cAMP-dependent protein kinase catalytic subunit beta antibody
- KAPCB_HUMAN antibody
see all
画像
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Western blot - Anti-PKA beta (catalytic subunit) antibody (ab187515)All lanes : Anti-PKA beta (catalytic subunit) antibody (ab187515) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PRKACB knockout HeLa cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : PRKACB knockout HAP1 cell lysate
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDaFalse colour image of Western blot: Anti-PKA beta (catalytic subunit) antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab187515 was shown to bind specifically to PKA beta (catalytic subunit). A band was observed at 41 kDa in wild-type HeLa cell lysates with no signal observed at this size in PRKACB knockout cell line ab264673 (knockout cell lysate ab258603). To generate this image, wild-type and PRKACB knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween� 20 (TBS-T) before incubation with primary antibodies overnight at 4 �C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-PKA beta (catalytic subunit) antibody (ab187515)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187515. Blue DAPI for nuclear staining. -
Western blot - Anti-PKA beta (catalytic subunit) antibody (ab187515)Anti-PKA beta (catalytic subunit) antibody (ab187515) at 1/500 dilution + SW480 cell extract
Predicted band size: 41 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab187515 は 7 報の論文で使用されています。
- Ye J et al. Tau inhibits PKA by nuclear proteasome-dependent PKAR2a elevation with suppressed CREB/GluA1 phosphorylation. Aging Cell 19:e13055 (2020). PubMed: 31668016
- Lv G et al. Inhibition of carrageenan-induced dental inflammatory responses owing to decreased TRPV1 activity by Dexmedetomidine. J Inflamm (Lond) 17:18 (2020). PubMed: 32377171
- Kurowska P et al. Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†. Biol Reprod 102:1290-1305 (2020). PubMed: 32149334
- Kurowska P et al. The role of vaspin in porcine corpus luteum. J Endocrinol 247:283-294 (2020). PubMed: 33108345
- Xue B et al. Astragaloside IV Protects Primary Cerebral Cortical Neurons from Oxygen and Glucose Deprivation/Reoxygenation by Activating the PKA/CREB Pathway. Neuroscience 404:326-337 (2019). PubMed: 30708047
- Qin R et al. Exercise training reduces ventricular arrhythmias through restoring calcium handling and sympathetic tone in myocardial infarction mice. Physiol Rep 7:e13972 (2019). PubMed: 30806037
- Zhang J et al. K-RasG12V/Y40C-PI3K/AKT pathway regulates H1.4S35ph through PKA to promote the occurrence and development of osteosarcoma cancer. Artif Cells Nanomed Biotechnol 47:2048-2057 (2019). PubMed: 31126199