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リコンビナントRabMAb

Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) 抗体 [EP2606Y] (ab75991)

Reviews (3)Q&A (1)References (59)
Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
  • Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
  • Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
  • Dot Blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
  • Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2606Y] to PKA alpha/beta/gamma (catalytic subunit) (phospho T197)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]
    PKA alpha/beta/gamma (catalytic subunit) 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2606Y] to PKA alpha/beta/gamma (catalytic subunit) (phospho T197)

  • 由来種

    Rabbit

  • 特異性

    This antibody recognizes the T197 phospho-site, which is the same site as the T198 on Uniprot P22612.

    Detects PKA alpha/beta/gamma (catalytic subunit) phosphorylated on threonine 197. Moreover, the antibody will cross-react with PRKX/Y (pT203).

  • アプリケーション

    適用あり: WB, IHC-P, Dot blotmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human PKA alpha/beta/gamma (catalytic subunit) aa 150-250 (phospho T197). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: HeLa, NIH/3T3, C6 whole cell lysate IHC: human liver carcinoma, mouse kidney, rat kidney

  • 特記事項

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75991の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000. Predicted molecular weight: 40 kDa.
IHC-P
1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary.
Dot blot
Use at an assay dependent concentration.
特記事項
WB
1/1000. Predicted molecular weight: 40 kDa.
IHC-P
1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary.
Dot blot
Use at an assay dependent concentration.

ターゲット情報

画像

  • Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution (Purified)

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes, whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
  • Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution (Unpurified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) 10% FBS all the time. Whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) serum starve for 24h then 20% FBS 20h. Whole cell lysates
    Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) serum starve for 24h then 20% FBS 20h.Whole cell lysates.Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 1 second


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Dot Blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Dot Blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)

    Dot blot analysis of various peptides labelling PKA gamma (catalytic subunit) (phospho T197) with unpurified ab75991 at a dilution of 1/1000. ab97051, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/100000).

    Lane 1 - PKA alpha/beta/gamma (catalytic subunit) (pT197) phospho peptide.

    Lane 2 - PKA alpha/beta/gamma (catalytic subunit) non-phospho peptide.

    Lane 3 - PRKX/Y (pT203) phospho peptide.

    Lane 4 - PRKX/Y non-phospho peptide.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)

参考文献 (59)

ab75991 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab75991 は 59 報の論文で使用されています。

  • Jin Y  et al. Effects of exercise-targeted hippocampal PDE-4 methylation on synaptic plasticity and spatial learning/memory impairments in D-galactose-induced aging rats. Exp Brain Res 242:309-320 (2024). PubMed: 38052997
  • Tian L  et al. C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages. iScience 26:107771 (2023). PubMed: 37731607
  • Hu L  et al. Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator. Proteome Sci 21:16 (2023). PubMed: 37821903
  • Xie L  et al. ITGB1 alleviates osteoarthritis by inhibiting cartilage inflammation and apoptosis via activating cAMP pathway. J Orthop Surg Res 18:849 (2023). PubMed: 37941009
  • Luo XD  et al. Activation of metabotropic glutamate receptor 1 regulates hippocampal CA1 region excitability in rats with status epilepticus by suppressing the HCN1 channel. Neural Regen Res 18:594-602 (2023). PubMed: 36018183
View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse brain tissue)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Mouse brain tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Hai-Ying Mary Cheng

Verified customer

投稿 Feb 13 2024

Western blot abreview for Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10)
Loading amount
20 µg
Treatment
100 µM IBMX
Specification
melanoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 06 2021

Western blot abreview for Anti-PKA gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (gel 10% SDS-PAGE)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Karine Thibault

Verified customer

投稿 Nov 05 2014

Question

This customer is highly interested in ab75991 (Anti-PKA gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]). This customer wants to know whether this antibody can specify recognize to the active form of PKA protein or not? Could you please help this customer to solve the problem? Thank for your kindly assistance. Best regards

Read More
Answer

Thank you for contacting us.

In GPCR signalling pathway cAMP binds to the regulatory subunit of PKA (Protein Kinase-A) leading to the dissociation of the catalytic subunits thereby making it active. PKA then lead to the phosphorylation of the GPCR for further cellular signalling.

Once phosphorylated PKA become active, this antibody detects the phophorylated form of PKA hence the active catalytic subunit of PKA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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