Anti-PKA alpha + beta (catalytic subunits) (phospho T197) 抗体 (ab59218)
Key features and details
- Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody
PKA alpha + beta (catalytic subunits) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197) -
由来種
Rabbit -
特異性
ab59218 detects endogenous levels of PKA alpha + beta (catalytic subunit) only when phosphorylated at threonine 197. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human PKA alpha + beta (catalytic subunits) aa 150-250 (phospho T197). (T-W-TP-L-C).
Database link: P17612 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab59218 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59218の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/100.
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| WB |
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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| 特記事項 |
|---|
|
IHC-P
1/50 - 1/100. |
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WB
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
ターゲット情報
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関連性
PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. -
細胞内局在
Cytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm -
参照データベース
- Entrez Gene: 5566 Human
- Entrez Gene: 5567 Human
- Entrez Gene: 18747 Mouse
- Entrez Gene: 18749 Mouse
- Entrez Gene: 25636 Rat
- Omim: 176892 Human
- Omim: 601639 Human
- SwissProt: P17612 Human
see all -
別名
- cAMP dependent protein kinase beta catalytic subunit antibody
- cAMP dependent protein kinase alpha catalytic subunit antibody
- cAMP dependent protein kinase catalytic subunit alpha antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)ab59218 at 1/50 dilution staining PKA alpha + beta (catalytic subunit) in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
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Western blot - Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)All lanes : Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218) at 1/500 dilution
Lane 1 : mouse brain cell extract
Lane 2 : mouse brain cell extract with immunising peptide
Predicted band size: 41 kDa
Observed band size: 41 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab59218 は 3 報の論文で使用されています。
- Peng H et al. Myomegalin regulates Hedgehog pathway by controlling PDE4D at the centrosome. Mol Biol Cell 32:1807-1817 (2021). PubMed: 34260267
- Bao Z et al. rTMS alleviates AD-induced cognitive impairment by inhibitng apoptosis in SAMP8 mouse. Aging (Albany NY) 13:26034-26045 (2021). PubMed: 34965216
- Skubal M et al. Altered splicing leads to reduced activation of CPEB3 in high-grade gliomas. Oncotarget 7:41898-41912 (2016). IHC . PubMed: 27256982