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Anti-PIWIL4/PIWI 抗体 (ab87939)

Reviews (1)Q&A (2)References (3)
Western blot - Anti-PIWIL4/PIWI antibody (ab87939)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL4/PIWI antibody (ab87939)

Key features and details

  • Rabbit polyclonal to PIWIL4/PIWI
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-PIWIL4/PIWI antibody
    PIWIL4/PIWI 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PIWIL4/PIWI

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to Human PIWIL4/PIWI.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab87939の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/200 - 1/1000. Predicted molecular weight: 97 kDa.
IHC-P
1/50 - 1/200.
特記事項
WB
1/200 - 1/1000. Predicted molecular weight: 97 kDa.
IHC-P
1/50 - 1/200.

ターゲット情報

  • 機能

    Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.

  • 組織特異性

    Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.

  • 配列類似性

    Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.

  • 翻訳後修飾

    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.

  • 細胞内局在

    Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DKFZp686P01248 antibody
      • FLJ36156 antibody
      • HILI 2 antibody
      • HILI2 antibody
      • HIWI 2 antibody
      • HIWI2 antibody
      • Miwi 2 protein antibody
      • Miwi2 antibody
      • PIWI antibody
      • Piwi like 2 antibody
      • Piwi like 4 (Drosophila) antibody
      • Piwi like 4 antibody
      • Piwi like protein 4 antibody
      • PIWI like protein antibody
      • Piwi like RNA mediated gene silencing 4 antibody
      • Piwi-like protein 4 antibody
      • PIWIL 4 antibody
      • Piwil4 antibody
      • PIWL4_HUMAN antibody
      see all

    画像

    • Western blot - Anti-PIWIL4/PIWI antibody (ab87939)
      Western blot - Anti-PIWIL4/PIWI antibody (ab87939)
      All lanes : Anti-PIWIL4/PIWI antibody (ab87939) at 1/500 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal testis cell lysate

      Predicted band size: 97 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL4/PIWI antibody (ab87939)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL4/PIWI antibody (ab87939)

      ab87939 at 1/100 dilution, staining PIWIL4/PIWI in formalin-fixed paraffin-embedded testis tissue showing nuclear staining.

    参考文献 (3)

    ab87939 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab87939 は 3 報の論文で使用されています。

    • Subhramanyam CS  et al. Role of PIWI-like 4 in modulating neuronal differentiation from human embryonal carcinoma cells. RNA Biol 17:1613-1624 (2020). PubMed: 32372724
    • Rocha-da-Silva L  et al. Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics. PLoS One 14:e0217941 (2019). PubMed: 31181099
    • Zhong F  et al. A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes. Nucleic Acids Res 43:10474-91 (2015). WB . PubMed: 26405199

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PIWIL4 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (CHO cells transiently overexpressing human PIWIL4)
    Permeabilization
    Yes - 0.1% Triton-X/PBS
    Specification
    CHO cells transiently overexpressing human PIWIL4
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 24 2017

    Question


    Trials 1-3 are my samples that I'm actually interested to see whether Piwil4 is expressed or not (so the answer is no). The positive mouse testis control I used was from Abcam, though unfortunately I don't know any further information regarding the lysate - it was from a former postdoc of our lab, aliquoted and only labeled as (mouse testis lysate/Abcam). I contacted him but he didn't remember whether its catalog number (obviously no lot number, either) was 4027 or 29289. I have to buy the lysate anyway, but just want to be sure what I'm purchasing is the right one - could you please check and give the lot number of ab29289 that is from fetal mice?
    Also, thank you for the precise sequence of the immunogen for Ab21869. Don't know how the homology was analyzed, but to me, it shows even higher homology than I checked last time with a broader region (aa.800-end). Please check out the attached file.
    Besides that, I also had a chance to try the antibody (21869) from one of my colleagues - unfortunately the result was pretty much the same - high background, and not detecting overexpressed-Piwil4. However, the person from whom I borrowed this 21869 antibody said that his previous batch was much better than this one (though he didn't do WB with these antibodies).

    So here is the bottom line. I'd like to have Ab87939 with a different lot number that I've been using. For Ab21869, I am willing to buy it myself, but want to try the lot number 15736 if possible. Can I order it in that way? Lastly, the same for the mouse testis lysate (ab29289) - please let me know whether there is any lysate lot from fetal mice. I'd like to buy the real 'positive' control.
    Thank you for your help,

    Read More
    Answer

    Thank you very much for your reply.
    We have two lots of ab29289 in stock, so I'll check with the lab whether either of these is from fetal mice. I will get back to you as soon as I have this information.
    I'll be happy to send a new lot of ab87939. Could you provide me with your original order or PO number, and I'll send this out promptly?
    Unfortunately we only have the same lot of ab21869 in stock.
    I look forward to hearing from you. If you have further questions or need anything else, please don't hesitate to ask.

    Read More

    Question

    Please see customers request below:

    I was wondering if the Abcam antibodies ab12337 and ab105393 (Piwil 1), ab85084 (Piwil 2) and ab87939 (Piwil 4) work on Platypus and Echidna. I would be doing Western blots and IHC with these antibodies. Also, if you have any information on any antibodies for Piwil 3 for the above applications and species, please pass them on. Please advise if we will be entitled to testing discounts as well.

    All going well, we would eventually want to do CHIP-Seq with these antibodies. Hence, if you have any information on that too, can you please pass them on.

    Please let me know if you require any further information.

    Thanks

    Kind regards,

    Read More
    Answer

    Thank you for contacting us.

    The sequence homology between human and Platypus species seems quite OK for antibodies ab12337 and ab85084 cross reactivity. These antibodies may be tried for further tests. We can provide abrreview discount for these products.

    Could you send us the required protein sequence of Echidna species for homology checks?

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

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