Anti-PIWIL4/PIWI 抗体 (ab180867)
Key features and details
- Rabbit polyclonal to PIWIL4/PIWI
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PIWIL4/PIWI antibody
PIWIL4/PIWI 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PIWIL4/PIWI -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human PIWIL4/PIWI aa 500-850. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q7Z3Z4 -
ポジティブ･コントロール
- HeLa, 231 and 293T cell extracts.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180867の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| WB |
1/500 - 1/2000. Predicted molecular weight: 97 kDa.
Fresh tissue samples is needed. |
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 97 kDa. Fresh tissue samples is needed. |
ターゲット情報
-
機能
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. -
組織特異性
Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed. -
配列類似性
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
翻訳後修飾
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. -
細胞内局在
Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization. - Information by UniProt
-
参照データベース
- Entrez Gene: 143689 Human
- Entrez Gene: 330890 Mouse
- Omim: 610315 Human
- SwissProt: Q7Z3Z4 Human
- SwissProt: Q8CGT6 Mouse
- Unigene: 660188 Human
- Unigene: 334969 Mouse
-
別名
- DKFZp686P01248 antibody
- FLJ36156 antibody
- HILI 2 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL4/PIWI antibody (ab180867)Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab180867. Blue DAPI for nuclear staining.
-
Western blot - Anti-PIWIL4/PIWI antibody (ab180867)All lanes : Anti-PIWIL4/PIWI antibody (ab180867) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : 231 cell extract
Lane 3 : 293T cell extract
Predicted band size: 97 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab180867 は 2 報の論文で使用されています。
- Liu J et al. piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification. Aging (Albany NY) 13:23361-23375 (2021). PubMed: 34645714
- He Z et al. PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat. J Virol 94:N/A (2020). PubMed: 32161174