Anti-Piwi 抗体 (ab5207)
Key features and details
- Rabbit polyclonal to Piwi
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
製品の概要
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製品名
Anti-Piwi antibody -
製品の詳細
Rabbit polyclonal to Piwi -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Drosophila melanogaster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in Drosophila embryo nuclear extract
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5207の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
追加情報
WB: 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Can be blocked with Piwi peptide (ab14803).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Piwi mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. It is expressed somatically in the ovariole terminal filament and epithelial sheath cells. Expressed in the germarium at low levels during oogenesis stages 1-6, at a lower level during stages 7-9, strongly at stage 10 and eventually accumulating in early embryos. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 34521 Drosophila melanogaster
- SwissProt: Q9VKM1 Drosophila melanogaster
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別名
- P-element induced wimpy testis antibody
- Piwi antibody
画像
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Western blot - Anti-Piwi antibody (ab5207)Anti-Piwi antibody (ab5207) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Piwi antibody (ab5207)This image is courtesy of Ahmet DenliDrosophila embryo lysate, 8% TRIS SDS-PAGE.
Drosophila embryo lysate, 8% TRIS SDS-PAGE. The arrow indicates Piwi at a predicted MW of 90 kDa. Pre-immune sera was used as a control and showed no equivalent band (data not shown). Ahmet Denli
The arrow indicates Piwi at a predicted MW of 90 kDa.
Pre-immune sera was used as a control and showed no equivalent band (data not shown).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab5207 は 8 報の論文で使用されています。
- Jiménez-Merino J et al. Putative stem cells in the hemolymph and in the intestinal submucosa of the solitary ascidian Styela plicata. Evodevo 10:31 (2019). PubMed: 31788180
- Jeffery WR Distal Regeneration Involves the Age Dependent Activity of Branchial Sac Stem Cells in the Ascidian Ciona intestinalis. Regeneration (Oxf) 2:1-18 (2015). PubMed: 25893097
- Patil VS et al. The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster. BMC Biol 12:61 (2014). WB ; Drosophila melanogaster . PubMed: 25287931
- Dufourt J et al. Spatio-temporal requirements for transposable element piRNA-mediated silencing during Drosophila oogenesis. Nucleic Acids Res N/A:N/A (2013). IHC-P ; Drosophila melanogaster . PubMed: 24288375
- Moshkovich N et al. RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. Genes Dev 25:1686-701 (2011). WB ; Drosophila melanogaster . PubMed: 21852534
- Klenov MS et al. Separation of stem cell maintenance and transposon silencing functions of Piwi protein. Proc Natl Acad Sci U S A 108:18760-5 (2011). PubMed: 22065765
- Brown FD et al. Whole body regeneration in a colonial ascidian, Botrylloides violaceus. J Exp Zool B Mol Dev Evol 312:885-900 (2009). PubMed: 19588490
- Rosner A et al. Vasa and the germ line lineage in a colonial urochordate. Dev Biol 331:113-28 (2009). WB . PubMed: 19406116