Anti-Pit1 抗体 [5E4] (ab10545)
Key features and details
- Mouse monoclonal [5E4] to Pit1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Rat
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Pit1 antibody [5E4]
Pit1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5E4] to Pit1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IPmore details -
種交差性
交差種: Rat
交差が予測される動物種: Sheep, Cow, Human, Pig, Macaque monkey -
免疫原
Recombinant fragment:
SAAECLPASN HATNVMSTAT GLHYSVPSCH YGNQPSTYGV MAGSLTPCLY KFPDHTLSHG FPPLHQPLLA EDPAASEFKQ ELRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKL
, corresponding to amino acids 30-146 of Rat Pit1. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
5E4 -
ミエローマ
NS1 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10545の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/40. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/40. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'. -
関連疾患
Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones. -
配列類似性
Belongs to the POU transcription factor family. Class-1 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 282315 Cow
- Entrez Gene: 5449 Human
- Entrez Gene: 25517 Rat
- Entrez Gene: 397325 Sheep
- Omim: 173110 Human
- SwissProt: P28069 Human
- SwissProt: Q04788 Pig
- SwissProt: P10037 Rat
see all -
別名
- Dwarf antibody
- GHF 1 antibody
- GHF 1A antibody
see all
画像
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At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1. At 1:40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.
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Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (ab10545)This image is courtesy of Zelton Dave Sharp
IF staining on rat samples using ab10545 carried out as per the reference (see below) by Zelton Dave Sharp of The University of Texas, San Antonio.
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Antibody to Pit1 (ab10545) detects a 33 kDa band in IP followed by WB.
Two IgG monoclonal antibodies were used to immunoprecipitate pituitary-derived GH3 cellular proteins, and followed by Western analysis. Whole cell extracts (WCE) and immunoprecipitated fractions contain a prominent 33 kD band representing Pit-1. Nonspecific hybridoma supernatant was used as a negative control. Epitope mapping (data not shown) indicated that both IgG monoclonal antibodies recognize an epitope in the distal N-terminus within the ST activation domain of Pit-1
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab10545 は 3 報の論文で使用されています。
- Huang Y et al. IRF1-mediated downregulation of PGC1a contributes to cardiorenal syndrome type 4. Nat Commun 11:4664 (2020). PubMed: 32938919
- Choi SY et al. Dipeptidyl peptidase-4 inhibitor gemigliptin protects against vascular calcification in an experimental chronic kidney disease and vascular smooth muscle cells. PLoS One 12:e0180393 (2017). WB ; Human . PubMed: 28686724
- Mancini MG et al. Subnuclear partitioning and functional regulation of the Pit-1 transcription factor. J Cell Biochem 72:322-38 (1999). PubMed: 10022514