Anti-Pit1 抗体 [2C11] (ab10623)
Key features and details
- Mouse monoclonal [2C11] to Pit1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Pit1 antibody [2C11]
Pit1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2C11] to Pit1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Sheep, Cow, Human, Pig, Macaque monkey -
免疫原
Recombinant fragment within Rat Pit1 aa 1-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
2C11 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10623の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
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特記事項 |
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WB
1/50. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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機能
Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'. -
関連疾患
Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones. -
配列類似性
Belongs to the POU transcription factor family. Class-1 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 282315 Cow
- Entrez Gene: 5449 Human
- Entrez Gene: 25517 Rat
- Entrez Gene: 397325 Sheep
- Omim: 173110 Human
- SwissProt: P28069 Human
- SwissProt: Q04788 Pig
- SwissProt: P10037 Rat
see all -
別名
- Dwarf antibody
- GHF 1 antibody
- GHF 1A antibody
see all
画像
データシートおよび資料
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Datasheet download
参考文献 (4)
ab10623 は 4 報の論文で使用されています。
- Ariyasu D et al. Decreased Activity of the Ghrhr and Gh Promoters Causes Dominantly Inherited GH Deficiency in Humanized GH1 Mouse Models. Endocrinology 160:2673-2691 (2019). PubMed: 31436800
- Zhang Z et al. MicroRNAs Regulate Pituitary Development, and MicroRNA 26b Specifically Targets Lymphoid Enhancer Factor 1 (Lef-1), Which Modulates Pituitary Transcription Factor 1 (Pit-1) Expression. J Biol Chem 285:34718-28 (2010). IHC-P ; Mouse . PubMed: 20807761
- Sharp ZD et al. Inactivating Pit-1 mutations alter subnuclear dynamics suggesting a protein misfolding and nuclear stress response. J Cell Biochem 92:664-78 (2004). PubMed: 15211565
- Mancini MG et al. Subnuclear partitioning and functional regulation of the Pit-1 transcription factor. J Cell Biochem 72:322-38 (1999). PubMed: 10022514