Anti-PIST 抗体 (ab37036)
Key features and details
- Rabbit polyclonal to PIST
- Suitable for: WB, IHC-P, ICC
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-PIST antibody
PIST 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PIST -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICCmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide of 16 amino acid from near the carboxy terminus of PIST (Human)
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ポジティブ･コントロール
- WB: Rat colon cell lysate. IHC-P: Rat colon tissue. ICC: Rat colon cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37036の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.
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| IHC-P |
Use a concentration of 1 µg/ml.
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| ICC |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa. |
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IHC-P
Use a concentration of 1 µg/ml. |
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ICC
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. -
組織特異性
Ubiquitously expressed. -
関連疾患
Note=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity. -
配列類似性
Contains 1 PDZ (DHR) domain. -
ドメイン
The PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1.
The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ. -
細胞内局在
Cytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. - Information by UniProt
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参照データベース
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別名
- CAL antibody
- CFTR associated ligand antibody
- CFTR-associated ligand antibody
see all
画像
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Western blot - Anti-PIST antibody (ab37036)Lane 1 : Anti-PIST antibody (ab37036) at 1 µg/ml
Lane 2 : Anti-PIST antibody (ab37036) at 2 µg/ml
Lane 3 : Anti-PIST antibody (ab37036) at 4 µg/ml
All lanes : rat colon cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody (ab37036)ab37036 at 1 µg/ml staining PIST in rat colon tissue by IHC.
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Immunocytochemistry - Anti-PIST antibody (ab37036)Immunocytochemical analysis of PIST in Rat Colon cells using ab37036 at 10 µg/mL.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab37036 は 7 報の論文で使用されています。
- Luo WY et al. PDZ Scaffold Protein CAL Couples with Metabotropic Glutamate Receptor 5 to Protect Against Cell Apoptosis and Is a Potential Target in the Treatment of Parkinson's Disease. Neurotherapeutics 16:761-783 (2019). PubMed: 31073978
- Li Y et al. A nonsense mutation in Ccdc62 gene is responsible for spermiogenesis defects and male infertility in repro29/repro29 mice. Biol Reprod 96:587-597 (2017). PubMed: 28339613
- Han F et al. Globozoospermia and lack of acrosome formation in GM130-deficient mice. Cell Death Dis 8:e2532 (2017). PubMed: 28055014
- Ohara N et al. Low expression of the GOPC is a poor prognostic marker in colorectal cancer. Oncol Lett 14:4483-4490 (2017). PubMed: 29085445
- Liu C et al. Sirt1 regulates acrosome biogenesis by modulating autophagic flux during spermiogenesis in mice. Development 144:441-451 (2017). PubMed: 28003215
- Wang H et al. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res 24:852-69 (2014). Mouse . PubMed: 24853953
- Xiao N et al. PICK1 deficiency causes male infertility in mice by disrupting acrosome formation. J Clin Invest 119:802-12 (2009). ICC/IF ; Rat, Mouse . PubMed: 19258705