Anti-Pirin/PIR 抗体 (ab21202)
Key features and details
- Goat polyclonal to Pirin/PIR
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Pirin/PIR antibody
Pirin/PIR 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Pirin/PIR -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human Pirin/PIR (N terminal).
Sequence:GSSKKVTLSVLSREC
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ポジティブ･コントロール
- Human heart lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21202の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 32 kDa).
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 32 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro). -
組織特異性
Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver. -
パスウェイ
Flavonoid metabolism; quercetin degradation. -
配列類似性
Belongs to the pirin family. -
細胞内局在
Nucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression. - Information by UniProt
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参照データベース
- Entrez Gene: 8544 Human
- Entrez Gene: 69656 Mouse
- Omim: 603329 Human
- SwissProt: O00625 Human
- SwissProt: Q9D711 Mouse
- Unigene: 495728 Human
- Unigene: 293463 Mouse
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別名
- 3-dioxygenase PIR antibody
- Iron binding nuclear protein antibody
- Pir antibody
see all
画像
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Western blot - Anti-Pirin/PIR antibody (ab21202)Anti-Pirin/PIR antibody (ab21202) at 0.3 µg/ml + Human Heart lysate (35µg protein in RIPA buffer).
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 30-35 kDa why is the actual band size different from the predicted?ab21202 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab21202 は 1 報の論文で使用されています。
- Brzóska K et al. Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element. Mol Cell Biochem 389:99-111 (2014). WB ; Human . PubMed: 24390086
