Anti-PIP5K3/PIKFYVE (phospho S307) 抗体 (ab62467)
Key features and details
- Rabbit polyclonal to PIP5K3/PIKFYVE (phospho S307)
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PIP5K3/PIKFYVE (phospho S307) antibody
PIP5K3/PIKFYVE 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PIP5K3/PIKFYVE (phospho S307) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human PIP5K3/PIKFYVE aa 250-350 (phospho S307).
Database link: Q9Y2I7 -
ポジティブ･コントロール
- Human brain tissue and HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab62467 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62467の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
|
ICC/IF
1/500 - 1/1000. |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes. -
関連疾患
Corneal dystrophy, fleck -
配列類似性
Contains 1 DEP domain.
Contains 1 FYVE-type zinc finger.
Contains 1 PIPK domain. -
細胞内局在
Endosome membrane. Mainly associated with membranes of the late endocytic pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 200576 Human
- Entrez Gene: 18711 Mouse
- Omim: 609414 Human
- SwissProt: Q9Y2I7 Human
- SwissProt: Q9Z1T6 Mouse
- Unigene: 744997 Human
- Unigene: 38370 Mouse
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別名
- 1 phosphatidylinositol 3 phosphate 5 kinase antibody
- 1 phosphatidylinositol 4 phosphate 5 kinase antibody
- 1-phosphatidylinositol 3-phosphate 5-kinase antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIP5K3/PIKFYVE (phospho S307) antibody (ab62467)
ab62467, at 1/50 dilution, staining PIP5K3/PIKFYVE in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-PIP5K3/PIKFYVE (phospho S307) antibody (ab62467)ab62467, at 1/500 dilution, staining PIP5K3/PIKFYVE in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab62467 は 1 報の論文で使用されています。
- Mei S et al. Disruption of PIKFYVE causes congenital cataract in human and zebrafish. Elife 11:N/A (2022). PubMed: 35023829