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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PIP5K1C 抗体 [MAO-R1] (ab109192)

Submit a review Q&A (1)References (9)
Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192)
  • Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192)
  • Anti-PIP5K1C antibody [MAO-R1] (ab109192)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MAO-R1] to PIP5K1C
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PIP5K1C antibody [MAO-R1]
    PIP5K1C 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [MAO-R1] to PIP5K1C

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, Neuro-2a, K562, C6 and 293T cell lysates; Human fetal kidney tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    MAO-R1

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109192の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 80-90 kDa (predicted molecular weight: 73 kDa).
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 80-90 kDa (predicted molecular weight: 73 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Plays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse. Mediates RAC1-dependent reorganization of actin filaments (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate.

  • 関連疾患

    Defects in PIP5K1C are the cause of lethal congenital contracture syndrome type 3 (LCCS3) [MIM:611369]; also known as multiple contractural syndrome Israeli Bedouin type B. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, the Pena-Shokeir phenotype, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS3 patients present at birth with severe multiple joint contractures with severe muscle wasting and atrophy, mainly in the legs. LCCS3 can be distinguished from the original LCCS by the absence of hydrops, fractures, and multiple pterygia.

  • 配列類似性

    Contains 1 PIPK domain.

  • 細胞内局在

    Cell membrane. Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Diphosphoinositide kinase antibody
    • LCCS3 antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type I gamma antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma antibody
    • PI51C antibody
    • PI51C_HUMAN antibody
    • PIP5K GAMMA antibody
    • PIP5K1-gamma antibody
    • Pip5k1c antibody
    • PIP5KIgamma antibody
    • PtdIns(4)P 5 kinase gamma antibody
    • PtdIns(4)P-5-kinase 1 gamma antibody
    • Type I PIP kinase antibody
    see all

画像

  • Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192)
    Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192)
    All lanes : Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PIP5K1C knockout HeLa cell lysate
    Lane 3 : Neuro-2a cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

    Predicted band size: 73 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab109192 observed at 73 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab109192 Anti-PIP5K1C antibody [MAO-R1] was shown to specifically react with PIP5K1C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265545 (knockout cell lysate ab258117) was used. Wild-type and PIP5K1C knockout samples were subjected to SDS-PAGE. ab109192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192)
    Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192)
    All lanes : Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Human fetal kidney cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : Neuro-2a cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 73 kDa

  • Anti-PIP5K1C antibody [MAO-R1] (ab109192)
    Anti-PIP5K1C antibody [MAO-R1] (ab109192)

参考文献 (9)

ab109192 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109192 は 9 報の論文で使用されています。

  • Yu M  et al. Targeting type Iγ phosphatidylinositol phosphate kinase overcomes oxaliplatin resistance in colorectal cancer. Theranostics 12:4386-4398 (2022). PubMed: 35673560
  • Gonzales B  et al. Type I Phosphatidylinositol-4-Phosphate 5-Kinases a and ? Play a Key Role in Targeting HIV-1 Pr55Gag to the Plasma Membrane. J Virol 94:N/A (2020). PubMed: 32376619
  • Ni W  et al. SUMOylation is required for PIPK1?-driven keratinocyte migration and growth. FEBS J 286:4709-4720 (2019). PubMed: 31276292
  • Wang DG  et al. PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism. Cell Rep 27:1991-2001.e5 (2019). PubMed: 31091439
  • Peng W  et al. Type I? phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer. EBioMedicine 44:375-386 (2019). PubMed: 31105034
  • Durand N  et al. The phosphorylation status of PIP5K1C at serine 448 can be predictive for invasive ductal carcinoma of the breast. Oncotarget 9:36358-36370 (2018). PubMed: 30555634
  • Jafari N  et al. p70S6K1 (S6K1)-mediated Phosphorylation Regulates Phosphatidylinositol 4-Phosphate 5-Kinase Type I ? Degradation and Cell Invasion. J Biol Chem 291:25729-25741 (2016). PubMed: 27780861
  • Tojima T  et al. Steering neuronal growth cones by shifting the imbalance between exocytosis and endocytosis. J Neurosci 34:7165-78 (2014). PubMed: 24849351
  • Chen C  et al. Integrin a9ß1 in airway smooth muscle suppresses exaggerated airway narrowing. J Clin Invest 122:2916-27 (2012). PubMed: 22772469

レビューと Q&A

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レビューを送る 質問を送る

Question

Our research interest is focused on the role of PIP2 in the regulation of secretory phase of cytotoxic event of human NK cells. We analysed with excellent results (Blood. 2008; 111(8):4165:4172) the contribution of distinct PIP5KIs-dependent PIP2 pool in NK cell functional activation. For our studies, we are interested in this product: anti-PIP5KIgamma antibody MAO-R1 catalog number ab 109192. As I read in the product's data sheet,  there are no references about this antibody.  Based on our experiences about lipids and PIP5Ks, we are available to test this antibody in order to provide a reference for your data sheet. In this regard, can we have a test sample? Please let me know if it's possible  

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Answer

Thank you for contacting us and for your interest in ab109192. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact us again with any other questions.  

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