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リコンビナントRabMAb

Anti-PINCH 1 + PINCH 2 抗体 [EP1943Y] (ab76112)

Submit a review Submit a question References (1)
Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1943Y] to PINCH 1 + PINCH 2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PINCH 1 + PINCH 2 antibody [EP1943Y]

  • 製品の詳細

    Rabbit monoclonal [EP1943Y] to PINCH 1 + PINCH 2

  • 由来種

    Rabbit

  • 特異性

    The immunogen used for this product shares 73% homology with PINCH 2. Cross-reactivity with this protein has not been confirmed experimentally.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: His-tagged Human full length recombinant LIMS1 and LIMS2 proteins, HeLa, Jurkat, HEK-293, and Mouse and Rat kidney lysate.

  • 特記事項

    This product has switched from a hybridoma to recombinant production method on 7th September 2023.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76112の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000.
特記事項
WB
1/1000 - 1/5000.

ターゲット情報

画像

  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
    Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
    All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/5000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 34 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
    Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
    All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 34 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
    Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
    All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/1000 dilution

    Lane 1 : His-tagged Human full length recombinant LIMS1 protein, 20 ng
    Lane 2 : His-tagged Human full length recombinant LIMS2 protein, 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 34 and 37 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking buffer and concentration: 5% NFDM/TBST. 

    Diluting buffer and concentration: 5% NFDM/TBST.

参考文献 (1)

ab76112 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76112 は 1 報の論文で使用されています。

  • Draicchio F  et al. Integrin-associated ILK and PINCH1 protein content are reduced in skeletal muscle of maintenance haemodialysis patients. J Physiol 598:5701-5716 (2020). PubMed: 32969494

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