Anti-Pin1 抗体 [EP1479Y] (ab76309)
![Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309)](https://www.abcam.co.jp/ps/products/76/ab76309/Images/ab76309-339725-ab76309WB1.jpg "Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1479Y] to Pin1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Pin1 antibody [EP1479Y]
Pin1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1479Y] to Pin1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa, HEK-293, Mouse brain and Rat brain lysates
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1479Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76309の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
For unpurified use at 1/500 |
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). For unpurified use at 1/500 |
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
ターゲット情報
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機能
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. -
配列類似性
Contains 1 PpiC domain.
Contains 1 WW domain. -
ドメイン
The WW domain is required for the interaction with STIL and KIF20B. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5300 Human
- Entrez Gene: 23988 Mouse
- Entrez Gene: 298696 Rat
- Omim: 601052 Human
- SwissProt: Q13526 Human
- SwissProt: Q9QUR7 Mouse
- Unigene: 465849 Human
- Unigene: 7906 Mouse
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別名
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
see all
画像
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Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309)All lanes : Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa -
![Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309)](https://www.abcam.co.jp/ps/products/76/ab76309/Images/ab76309-91699-anti-pin1-antibody-ep1479y-western-blot.jpg)
Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309)Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + HEK-293 cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa -
![Anti-Pin1 antibody [EP1479Y] (ab76309)](https://www.abcam.co.jp/ps/products/76/ab76309/Images/ab76309-5-benefits-of-recombinant-antibodies.png)
Anti-Pin1 antibody [EP1479Y] (ab76309)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab76309 は 7 報の論文で使用されています。
- Mishra M et al. Adaptor Protein p66Shc: A Link Between Cytosolic and Mitochondrial Dysfunction in the Development of Diabetic Retinopathy. Antioxid Redox Signal 30:1621-1634 (2019). PubMed: 30105917
- Nechama M et al. The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation. Nat Commun 9:1603 (2018). PubMed: 29686383
- Xu T et al. Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis. Neoplasia 19:216-225 (2017). PubMed: 28167297
- Ezratty EJ et al. A Presenilin-2-ARF4 trafficking axis modulates Notch signaling during epidermal differentiation. J Cell Biol 214:89-101 (2016). PubMed: 27354375
- Guito J et al. The cellular peptidyl-prolyl cis/trans isomerase Pin1 regulates reactivation of Kaposi's sarcoma-associated herpesvirus from latency. J Virol 88:547-58 (2014). IP . PubMed: 24173213
- Ma SL et al. Prolyl isomerase Pin1 promotes amyloid precursor protein (APP) turnover by inhibiting glycogen synthase kinase-3ß (GSK3ß) activity: novel mechanism for Pin1 to protect against Alzheimer disease. J Biol Chem 287:6969-73 (2012). IP ; Human . PubMed: 22184106
- Krishnan N et al. The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination. Mol Cell Biol 32:4306-22 (2012). PubMed: 22907757