Anti-PIM1 抗体 [EPR24447-39A] - BSA and Azide free (Detector) (ab300780)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24447-39A] to PIM1 - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse, Human
製品の概要
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製品名
Anti-PIM1 antibody [EPR24447-39A] - BSA and Azide free (Detector)
PIM1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24447-39A] to PIM1 - BSA and Azide free (Detector) -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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特記事項
Ab300780 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human, mouse PIM1 . The recommended pair for sandwich ELISA is:
Capture: ab300779, human, mouse PIM1 Capture Antibody (unconjugated)
Detector: ab300780, human, mouse PIM1 Detector Antibody (unconjugated)
The reference range value is 78-5000 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24447-39A -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300780の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24447-38] to PIM1 - BSA and Azide free (Capture) (ab300779).
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特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24447-38] to PIM1 - BSA and Azide free (Capture) (ab300779). |
ターゲット情報
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機能
May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. -
組織特異性
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on both serine/threonine and tyrosine residues. -
細胞内局在
Cytoplasm. Nucleus and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5292 Human
- Entrez Gene: 18712 Mouse
- Omim: 164960 Human
- SwissProt: P11309 Human
- SwissProt: P06803 Mouse
- Unigene: 81170 Human
- Unigene: 405293 Mouse
- Unigene: 485038 Mouse
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別名
- Oncogene PIM 1 antibody
- Oncogene PIM1 antibody
- PIM 1 antibody
see all
画像
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Sandwich ELISA of ab300778 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab300780 は論文での使用が確認できていません。