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リコンビナントRabMAb

Anti-PIM1 抗体 [EPR24447-39] - BSA and Azide free (ab300454)

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Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
  • Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
  • Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
  • Immunoprecipitation - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
  • Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24447-39] to PIM1
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free
    PIM1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24447-39] to PIM1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WBmore details
    適用なし: Flow Cyt (Intra) or IHC-P

  • 種交差性

    交差種: Mouse, Human
    非交差種: Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: K-562, K-562 transfected with siRNA specifically targeti PIM1, whole, K-562, A20, F9, His-tagged human PIM1 recombinant protein and His-tagged human PIM3 recombinant protein lysates. IP: A20 cell.

  • 特記事項

    ab300454 is the carrier-free version of ab300453.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300454の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra) or IHC-P.

ターゲット情報

  • 機能

    May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.

  • 組織特異性

    Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.

  • 配列類似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Autophosphorylated on both serine/threonine and tyrosine residues.

  • 細胞内局在

    Cytoplasm. Nucleus and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Oncogene PIM 1 antibody
      • Oncogene PIM1 antibody
      • PIM 1 antibody
      • pim 1 kinase 44 kDa isoform antibody
      • Pim 1 kinase antibody
      • pim 1 oncogene (proviral integration site 1) antibody
      • Pim 1 oncogene antibody
      • PIM antibody
      • PIM1 antibody
      • pim1 kinase 44 kDa isoform antibody
      • PIM1_HUMAN antibody
      • Pim2 antibody
      • PIM3 antibody
      • Proto oncogene serine/threonine protein kinase Pim 1 antibody
      • Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
      • Proviral integration site 1 antibody
      • Proviral integration site 2 antibody
      see all

    画像

    • Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
      Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
      All lanes : Anti-PIM1 antibody [EPR24447-39] (ab300453) at 1/5000 dilution

      Lane 1 : His-tagged human PIM1 recombinant protein
      Lane 2 : His-tagged human PIM3 recombinant protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 34,44 kDa why is the actual band size different from the predicted?


      Exposure time: 7 seconds


      This data was developed using ab300453, the same antibody clone in a different buffer formulation. 

      Blocking / Diluting buffer and concentration:5% NFDM/TBST

       

    • Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
      Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
      All lanes : Anti-PIM1 antibody [EPR24447-39] (ab300453) at 1/1000 dilution

      Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
      Lane 2 : A20 (Mouse reticulum sarcoma B lymphocyte), whole cell lysate
      Lane 3 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 34 kDa why is the actual band size different from the predicted?



      This data was developed using ab300453, the same antibody clone in a different buffer formulation. 

      Blocking / Diluting buffer and concentration:5% NFDM/TBST

      Exposure time:

      Lane 1, 2: 70 seconds
      Lane 3: 3 minutes

    • Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
      Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
      All lanes : Anti-PIM1 antibody [EPR24447-39] (ab300453) at 1/1000 dilution

      Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control, whole cell lysate
      Lane 2 : K-562 transfected with siRNA specifically targeting PIM1, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 34 kDa why is the actual band size different from the predicted?


      Exposure time: 37 seconds


      This data was developed using ab300453, the same antibody clone in a different buffer formulation. 

      Blocking / Diluting buffer and concentration:5% NFDM/TBST

       

    • Immunoprecipitation - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
      Immunoprecipitation - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)

      This data was developed using ab300453, the same antibody clone in a different buffer formulation.

      PIM1 was immunoprecipitated from 0.35 mg A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 ug with ab300453 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300453 at ID63 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 ug

      Lane 2: ab300453 IP in A20 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300454 in A20 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

    • Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
      Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300454 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300454 は論文での使用が確認できていません。

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