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AB21271

Anti-PIKE 抗体

Anti-PIKE antibody

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(1 Publication)

Rabbit Polyclonal PIKE antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human AGAP2.

別名を表示する

CENTG1, KIAA0167, AGAP2, AGAP-2, Centaurin-gamma-1, GTP-binding and GTPase-activating protein 2, Phosphatidylinositol 3-kinase enhancer, Cnt-g1, GGAP2, PIKE

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIKE antibody (AB21271)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIKE antibody (AB21271)

ab21271 at 10μg/ml staining PIKE in mouse brain tissue by IHC

Western blot - Anti-PIKE antibody (AB21271)
  • WB

Unknown

Western blot - Anti-PIKE antibody (AB21271)

ab21271 at concentrations of 0.5 (lane A), 1 (lane B) and 2 μg/ml (lane C), staining PIKE-L and PIKE-A in mouse brain cell lysates by Western blot.

Lane 1:

Western blot - Anti-PIKE antibody (ab21271) at 0.5 µg/mL

Lane 2:

Western blot - Anti-PIKE antibody (ab21271) at 1 µg/mL

Lane 3:

Western blot - Anti-PIKE antibody (ab21271) at 2 µg/mL

All lanes:

Mouse brain cell lysate

Predicted band size: 124 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human AGAP2. The exact immunogen used to generate this antibody is proprietary information.

Q99490

特異性

Recognises PIKE: also detect both PIKE-L and PIKE-A isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Purified by ion exchange chromatography.
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PIKE also known as phosphoinositide 3-kinase enhancer is a protein encoded by the CENTG1 gene. It weighs approximately 97 kDa and has several isoforms including PIKE-A PIKE-L and PIKE-S each with distinct localizations and functions. PIKE is expressed in neural tissues especially in the brain where it regulates numerous signaling processes. This protein facilitates the activation of phosphoinositide 3-kinase (PI3K) by directly interacting with it thereby enhancing downstream signaling.
Biological function summary

Proteins like PIKE are important in regulating cellular growth survival and proliferation. PIKE acts as an important mediator in the PI3K signaling pathway and is known to affect neuronal cell development and neurite outgrowth. It participates in forming complexes with other proteins such as Akt further amplifying signaling cascades linked to cell survival and growth. Its prominent role in neural systems makes PIKE significant in maintaining normal brain function.

Pathways

The protein PIKE integrates significantly into the PI3K/Akt signaling pathway and the small GTPase pathway. In the PI3K/Akt pathway PIKE works closely with Akt and mTOR proteins promoting cell survival and growth. In the small GTPase pathway PIKE interacts with proteins like Rac1 which regulates actin cytoskeleton dynamics and influences changes in cell shape and motility. These pathways highlight PIKE's involvement in essential cellular processes.

The abnormal regulation of PIKE links to specific conditions such as cancer and schizophrenia. In cancer altered PIKE activity contributes to tumorigenesis through its interaction with growth-related proteins including Akt. In schizophrenia changes in PIKE expression or function can affect neural signaling pathways potentially influencing cognitive and behavioral symptoms associated with the disorder. Understanding PIKE's role in these conditions could aid in developing new therapeutic strategies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
See full target information AGAP2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer genomics & proteomics 21:485-501 PubMed39191501

2024

DNA Methylation in Recurrent Glioblastomas: Increased TEM8 Expression Activates the Src/PI3K/AKT/GSK-3β/B-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Paramita Kundu,Ruchi Jain,Nandaki Nag Kanuri,Arivazhagan Arimappamagan,Vani Santosh,Paturu Kondaiah
View all publications
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