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AB201693

Anti-PIGK 抗体 [EPR17843]

Anti-PIGK antibody [EPR17843]

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(10 Publications)

Rabbit Recombinant Monoclonal PIGK antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

別名を表示する

GPI8, PIGK, GPI-anchor transamidase, GPI8 homolog, Phosphatidylinositol-glycan biosynthesis class K protein, hGPI8, PIG-K

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-PIGK antibody [EPR17843] (AB201693)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PIGK antibody [EPR17843] (AB201693)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PIGK with ab201693 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing cytoplasmic staining on Jurkat cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :

-ve control 1 : ab201693 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Flow Cytometry (Intracellular) - Anti-PIGK antibody [EPR17843] (AB201693)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PIGK antibody [EPR17843] (AB201693)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HEK-293 (Human epithelial cells from embryonic kidney) cells labeling PIGK with ab201693 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730;black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-PIGK antibody [EPR17843] (AB201693)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PIGK antibody [EPR17843] (AB201693)

Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling PIGK with ab201693 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing cytoplasmic staining on A549 cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :

-ve control 1 : ab201693 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunoprecipitation - Anti-PIGK antibody [EPR17843] (AB201693)
  • IP

Supplier Data

Immunoprecipitation - Anti-PIGK antibody [EPR17843] (AB201693)

PIGK was immunoprecipitated from 1mg of HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab201693 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab201693 at 1/2000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : HEK293 whole cell lysate10 μg (Input).

Lane 2 : ab201693 IP in HEK293 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab201693 in HEK293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-PIGK antibody [EPR17843] (ab201693)

Predicted band size: 45 kDa

false

Western blot - Anti-PIGK antibody [EPR17843] (AB201693)
  • WB

Supplier Data

Western blot - Anti-PIGK antibody [EPR17843] (AB201693)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PIGK antibody [EPR17843] (ab201693) at 1/2000 dilution

Lane 1:

HT1080 (Human fibrosarcoma cells) cell lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Exposure time: 1min

Western blot - Anti-PIGK antibody [EPR17843] (AB201693)
  • WB

Supplier Data

Western blot - Anti-PIGK antibody [EPR17843] (AB201693)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PIGK antibody [EPR17843] (ab201693) at 1/2000 dilution

Lane 1:

Human pancreas lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Exposure time: 3min

Western blot - Anti-PIGK antibody [EPR17843] (AB201693)
  • WB

Supplier Data

Western blot - Anti-PIGK antibody [EPR17843] (AB201693)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PIGK antibody [EPR17843] (ab201693) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat kidney lysate at 10 µg

Lane 7:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Exposure time: 1min

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-PIGK antibody [EPR17843] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17843

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

IP, Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Primary Antibodies

AB190693

Anti-AER61 antibody [EPR12944]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-AER61 antibody [EPR12944] (AB190693)

  • 0
  • 0
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
  • 28
Primary Antibodies

AB192255

Anti-Pigu antibody [EPR16424] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pigu antibody [EPR16424] - C-terminal (AB192255)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PIGK also known as phosphatidylinositol glycan anchor biosynthesis class K functions as an essential component of the glycosylphosphatidylinositol (GPI) transamidase complex. This protein carries a mass of approximately 37 kDa. You find PIGK in the endoplasmic reticulum across various cell types where it assists in the post-translational modification processes required for protein anchoring to cell membranes.
Biological function summary

The GPI transamidase complex which includes PIGK facilitates the attachment of proteins to the cell membrane via GPI anchors. This activity is a critical post-translational modification essential for the functionality and localization of these proteins. Within this complex PIGK operates alongside other components such as PIGT and GPAA1 to ensure correct positioning and attachment of GPI-anchored proteins.

Pathways

PIGK contributes to the biosynthesis and remodeling of GPI-anchored proteins impacting the GPI-anchor biosynthesis pathway itself. This pathway plays an integral role in cellular processes that involve anchoring proteins like CD55 and CD59 which are important for membrane dynamics and cell surface interactions. As part of these pathways PIGK's function links closely with other proteins in the remodeling process ensuring proper protein placement and functionality.

PIGK's role in GPI-anchor biosynthesis links it to various inherited GPI deficiencies. A disruption in PIGK function may contribute to neurological symptoms and developmental disorders like hyperphosphatasia with mental retardation syndrome (HPMRS). The connection between PIGK and diseases often involves interactions with other components of the GPI biosynthesis pathway such as PIGA highlighting the significance of PIGK in maintaining cellular and physiological homeostasis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalytic subunit of the glycosylphosphatidylinositol-anchor (GPI-anchor) transamidase (GPI-T) complex that catalyzes the formation of the linkage between a proprotein and a GPI-anchor and participates in GPI anchored protein biosynthesis (PubMed : 10793132, PubMed : 11483512, PubMed : 12582175, PubMed : 34576938, PubMed : 35165458, PubMed : 35551457, PubMed : 37684232, PubMed : 9356492). Recognizes diverse proproteins at a C-terminal signal peptide (CSP) region that lacks consensus sequence and replaces it with a GPI-anchor via a transamidation reaction (PubMed : 35165458, PubMed : 35551457, PubMed : 37684232). Transamidation catalysis reaction follows a two-phase mechanism (PubMed : 37684232). In the acyl-enzyme phase, the carbonyl group of the proproteins's omega-site undergoes a nucleophilic attack forming an enzyme-substrate thioester bond (PubMed : 37684232). Followed by a general acid catalysis that allows CSP releasing, regenerating the carbonyl, and forming the acyl-enzyme intermediate (PubMed : 37684232). In the GPI-anchor attachment phase, the amino group of the GPI-anchor's ethanolamine phosphate, the one on third mannose (EtNP3), mediates a nucleophilic attack on the carbonyl of the acyl-enzyme intermediate, replacing the CSP, allowing GPI-anchor attachment to the omega-residue, therefore forming the product and freeing the enzyme (PubMed : 37684232).
See full target information PIGK
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e02838 PubMed40548932

2025

CD74 Blockade Disrupts Endothelial Migrasome Signaling to Prevent Inflammatory Macrophage Differentiation and Inhibit Atherosclerotic Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kangnan Zhang,Jiong Chen,Zhenhua Zhu,Hong Hu,Qinghui Zhang,Rongrong Jia,Na Wang,Shihao Xiang,Yong Zhou,Yuehong Wang,Ling Xu

Cancer biology & medicine 22: PubMed40488414

2025

Cancer cell-derived migrasomes harboring ATF6 promote breast cancer brain metastasis endoplasmic reticulum stress-mediated disruption of the blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Song Wang,Guohao Gu,Xinmiao Xian,Jun Li,Di Zhang,Jianran Guo,Anqi Zhang,Shen Chen,Dong Yan,Bingwu Yang,Meng An,Wei Zhang,Bo Fu

Journal of extracellular vesicles 13:e12460 PubMed38853287

2024

Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Yang,Heng Zhang,Si Chen,Kaibin Lou,Meng Zhou,Mingchao Zhang,Rui Lu,Chunxia Zheng,Limin Li,Qihan Chen,Zhihong Liu,Ke Zen,Yanggang Yuan,Hongwei Liang

Journal of experimental & clinical cancer research : CR 43:160 PubMed38840183

2024

CD151-enriched migrasomes mediate hepatocellular carcinoma invasion by conditioning cancer cells and promoting angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kangnan Zhang,Zhenhua Zhu,Rongrong Jia,N A Wang,Min Shi,Yugang Wang,Shihao Xiang,Qinghui Zhang,Ling Xu

Current protocols 3:e856 PubMed37540780

2023

Detection, Purification, Characterization, and Manipulation of Migrasomes.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Jiang,Ying Li,Li Yu

The Journal of cell biology 222: PubMed37389656

2023

CLPTM1L is a GPI-anchoring pathway component targeted by HCMV.

Applications

Unspecified application

Species

Unspecified reactive species

Inbal Kol,Ahmed Rishiq,Mevaseret Cohen,Shira Kahlon,Ophir Pick,Liat Dassa,Natan Stein,Yotam Bar-On,Dana G Wolf,Einat Seidel,Ofer Mandelboim

The FEBS journal 290:3359-3368 PubMed36808246

2023

Isolation and characterization of extracellular vesicle-like nanoparticles derived from migrasomes.

Applications

Unspecified application

Species

Unspecified reactive species

Yongbin Ma,Tao Li,Leyu Zhao,Dan Zhou,Liyang Dong,Zhonghua Xu,Yu Wang,Xin Yao,Kai Zhao

Frontiers in cell and developmental biology 9:801687 PubMed35096829

2022

Identification and Validation of Key Genes of Differential Correlations in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tingna Chen,Qiuming He,Zhenxian Xiang,Rongzhang Dou,Bin Xiong

Cell structure and function 46:65-71 PubMed34193731

2021

Hrd1-dependent Degradation of the Unassembled PIGK Subunit of the GPI Transamidase Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Kohei Kawaguchi,Miki Yamamoto-Hino,Yoshiko Murakami,Taroh Kinoshita,Satoshi Goto

Kidney diseases (Basel, Switzerland) 6:422-433 PubMed33313063

2020

Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Shan Li,Weiwei Rong,Caihong Zeng,Xiaodong Zhu,Qilin Chen,Limin Li,Zhi-Hong Liu,Ke Zen
View all publications
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