Anti-PIG3 抗体 (ab64798)
Key features and details
- Rabbit polyclonal to PIG3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PIG3 antibody
PIG3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PIG3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Chimpanzee, Rhesus monkey
-
免疫原
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human PIG3.
Immunogen の所有権に関して (Peptide available as ab68589.) -
ポジティブ･コントロール
- Recombinant Human PIG3 protein (ab95857) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Caco2, A549, WI-38, SW480, TE671, Human Lung Tissue, Human Skeletal Muscle Tissue, Human Colon Tissue
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64798の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
ターゲット情報
-
機能
May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. Has low NADPH-dependent diamine reductase activity (in vitro). -
配列類似性
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. - Information by UniProt
-
参照データベース
- Entrez Gene: 9540 Human
- Entrez Gene: 704206 Rhesus monkey
- Omim: 605171 Human
- SwissProt: Q53FA7 Human
- Unigene: 50649 Human
-
別名
- p53 induced gene 3 protein antibody
- p53-induced gene 3 protein antibody
- Putative quinone oxidoreductase antibody
see all
画像
-
Western blot - Anti-PIG3 antibody (ab64798)All lanes : Anti-PIG3 antibody (ab64798) at 1 µg/ml
Lane 1 : Caco-2 whole cell lysate (ab3950)
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 6 : Lung (Human) Tissue Lysate
Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 8 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 2 minutes
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab64798 は 4 報の論文で使用されています。
- Ryan SL et al. Identification of Proteins Deregulated by Platinum-Based Chemotherapy as Novel Biomarkers and Therapeutic Targets in Non-Small Cell Lung Cancer. Front Oncol 11:615967 (2021). PubMed: 33777753
- Mungamuri SK et al. Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene 0:N/A (2014). PubMed: 25023704
- Kracikova M et al. A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ 20:576-88 (2013). PubMed: 23306555
- Mungamuri SK et al. p53-mediated heterochromatin reorganization regulates its cell fate decisions. Nat Struct Mol Biol 19:478-84, S1 (2012). WB . PubMed: 22466965