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リコンビナントRabMAb

Anti-PICK1 抗体 [EPR25155-48] - BSA and Azide free (ab290742)

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Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
  • Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
  • Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
  • Immunocytochemistry/ Immunofluorescence - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
  • Flow Cytometry (Intracellular) - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
  • Immunoprecipitation - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
  • Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25155-48] to PICK1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt (Intra), IP
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free
    PICK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25155-48] to PICK1 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    Please note that this antibody does not react with Human species for ICC and FC application and does not react with Mouse species for IP application.

  • アプリケーション

    適用あり: ICC/IF, WB, Flow Cyt (Intra), IPmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: C2C12 (mouse myoblasts myoblast) and HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates. ICC/IF: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) Flow Cyt (Intra): RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage). IP:LNCaP (human prostate carcinoma epithelial cell) whole cell lysate.

  • 特記事項

    ab290742 is the carrier-free version of ab290727.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290742の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Contains 1 AH domain.
    Contains 1 PDZ (DHR) domain.

  • 翻訳後修飾

    Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.

  • 細胞内局在

    Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
  • Information by UniProt

  • 参照データベース

    • 別名

      • dJ1039K5 antibody
      • MGC15204 antibody
      • OTTHUMP00000028509 antibody
      • PICK 1 antibody
      • Pick1 antibody
      • PICK1_HUMAN antibody
      • PRKCA binding protein antibody
      • PRKCA-binding protein antibody
      • PRKCABP antibody
      • Protein interacting with C kinase 1 antibody
      • Protein interacting with PRKCA antibody
      • Protein interacting with PRKCA 1 antibody
      • Protein kinase C alpha binding protein antibody
      • Protein kinase C-alpha-binding protein antibody
      • Protein that interacts with C kinase 1 antibody
      see all

    画像

    • Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      Anti-PICK1 antibody [EPR25155-48] (ab290727) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 46 kDa
      Observed band size: 46 kDa


      Exposure time: 3 minutes


      This data was developed using ab290727, the same antibody clone in a different buffer formulation.

      Blocking / Dilution buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID 10027300;PMID: 32336285).

    • Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      All lanes : Anti-PICK1 antibody [EPR25155-48] (ab290727) at 1/1000 dilution

      Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control, whole cell lysate
      Lane 2 : 293T transfected with siRNA specifically targeting PICK1, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 46 kDa
      Observed band size: 46 kDa


      Exposure time: 3 minutes


      This data was developed using ab290727, the same antibody clone in a different buffer formulation.

      Blocking / Dilution buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID 10027300;PMID: 32336285).

    • Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      Western blot - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      All lanes : Anti-PICK1 antibody [EPR25155-48] (ab290727) at 1/1000 dilution

      Lane 1 : C2C12 (mouse myoblasts myoblast) whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 46 kDa
      Observed band size: 46 kDa


      Exposure time: 3 minutes


      This data was developed using ab290727, the same antibody clone in a different buffer formulation. 

      Blocking / Diluent buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID 10027300;PMID: 32336285).

    • Immunocytochemistry/ Immunofluorescence - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      Immunocytochemistry/ Immunofluorescence - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)

      This data was developed using ab290727, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells lebelling PICK1 with ab290727 at 1/50 (10.2 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/mL) dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/mL) dilution.

    • Flow Cytometry (Intracellular) - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      Flow Cytometry (Intracellular) - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)

      This data was developed using ab290727, the same antibody clone in a different buffer formulation.

      Intracellular Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling PICK1 with ab290727 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      Immunoprecipitation - Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)

      This data was developed using ab290727, the same antibody clone in a different buffer formulation.

      PICK1 was immunoprecipitated from 0.35 mg LNCaP (human prostate carcinoma epithelial cell) whole cell lysate 10 ug with ab290727 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290727 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate 10 ug

      Lane 2: ab290727 IP in LNCaP whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab290727 in LNCaP whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

    • Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)
      Anti-PICK1 antibody [EPR25155-48] - BSA and Azide free (ab290742)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290742 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290742 は論文での使用が確認できていません。

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