Anti-PICK1 抗体 (ab3420)
Key features and details
- Rabbit polyclonal to PICK1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PICK1 antibody
PICK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PICK1 -
由来種
Rabbit -
特異性
Detects protein interacting with Protein Kinase C (PICK 1). By Western blot, this antibody detects an ~52 kDa protein representing PICK 1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK 1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Cynomolgus monkey
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免疫原
Synthetic peptide corresponding to Mouse PICK1 aa 1-100.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3420の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa.
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| ICC/IF | (3) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 AH domain.
Contains 1 PDZ (DHR) domain. -
翻訳後修飾
Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors. -
細胞内局在
Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses. - Information by UniProt
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参照データベース
- Entrez Gene: 9463 Human
- Entrez Gene: 18693 Mouse
- Entrez Gene: 84591 Rat
- Omim: 605926 Human
- SwissProt: Q2T9M1 Cow
- SwissProt: Q9NRD5 Human
- SwissProt: Q62083 Mouse
- SwissProt: Q9EP80 Rat
see all -
別名
- dJ1039K5 antibody
- MGC15204 antibody
- OTTHUMP00000028509 antibody
see all
画像
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Western blot - Anti-PICK1 antibody (ab3420)This image is courtesy of an anonymous abreview.Lane 1 : An myc antibody in PBST for 16 hours at 4°C
Lane 2 : Anti-PICK1 antibody (ab3420) at 1/500 dilution (in PBST for 16 hours at 4°C)
All lanes : Transfected CHO cells expressing human Pick1 tagged with myc
Lysates/proteins at 20 µg per lane.
Secondary
Lane 2 : An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: ~52 kDa why is the actual band size different from the predicted?
Additional bands at: ~40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at 20°C -
Immunocytochemistry/ Immunofluorescence - Anti-PICK1 antibody (ab3420)This image is courtesy of an anonymous abreview.ab3420 staining PICK1 in mouse Hippocampal neurons by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% saponin/PBS prior to blocking in 3% BSA for 1 hour at room temperature. The primary antibody was diluted 1/300 in 3% BSA, 0.1% saponin /PBS and incubated with the sample for 18 hours at 4 °C. The secondary antibody was Alexa Fluor® 455-conjugated Donkey anti-Rabbit polyclonal, diluted 1/1000.
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Immunocytochemistry/ Immunofluorescence - Anti-PICK1 antibody (ab3420)This image is courtesy of an anonymous abreview.ab3420 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500. -
Western blot - Anti-PICK1 antibody (ab3420)Western blot of PICK1 on AtT20 cell lysate using ab3420.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (18)
ab3420 は 18 報の論文で使用されています。
- Lee DS et al. PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus. Sci Rep 13:13927 (2023). PubMed: 37626185
- Binkle L et al. The adaptor protein PICK1 targets the sorting receptor SorLA. Mol Brain 15:18 (2022). PubMed: 35183222
- Pardo M et al. Heterozygote Dopamine Transporter Knockout Rats Display Enhanced Cocaine Locomotion in Adolescent Females. Int J Mol Sci 23:N/A (2022). PubMed: 36499749
- Kim JE et al. Inhibition of AKT/GSK3ß/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats. Biomedicines 9:N/A (2021). PubMed: 33919872
- Du JJ et al. Clathrin-independent but dynamin-dependent mechanisms mediate Ca2+-triggered endocytosis of the glutamate GluK2 receptor upon excitotoxicity. J Integr Neurosci 19:449-458 (2020). PubMed: 33070524
