Anti-PIAS3 抗体 (ab58406)
Key features and details
- Rabbit polyclonal to PIAS3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PIAS3 antibody
PIAS3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PIAS3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide derived from Internal of human PIAS3.
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ポジティブ･コントロール
- Human lung carcinoma tissue 293 cells treated with UV (5 mins)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58406の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376). -
組織特異性
Widely expressed. -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger. -
ドメイン
The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
The LXXLL motif is a transcriptional coregulator signature. -
翻訳後修飾
Sumoylated. -
細胞内局在
Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules. - Information by UniProt
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参照データベース
- Entrez Gene: 10401 Human
- Entrez Gene: 229615 Mouse
- Entrez Gene: 83614 Rat
- Omim: 605987 Human
- SwissProt: Q9Y6X2 Human
- SwissProt: O54714 Mouse
- SwissProt: O70260 Rat
- Unigene: 435761 Human
see all -
別名
- E3 SUMO protein ligase PIAS 3 antibody
- E3 SUMO protein ligase PIAS3 antibody
- E3 SUMO-protein ligase PIAS3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS3 antibody (ab58406)Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab58406 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
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Western blot - Anti-PIAS3 antibody (ab58406)All lanes : Anti-PIAS3 antibody (ab58406) at 1/500 dilution
Lane 1 : 293 cell extract treated with UV (5 min)
Lane 2 : 293 cell extract treated with UV (5 min) with peptide
Predicted band size: 67 kDa
Observed band size: 67 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab58406 は 6 報の論文で使用されています。
- Zhao Q et al. LncRNA FOXD2-AS1 promotes cell proliferation and invasion of fibroblast-like synoviocytes by regulation of miR-331-3p/PIAS3 pathway in rheumatoid arthritis. Autoimmunity 54:254-263 (2021). PubMed: 34030529
- Sun H et al. PIAS3/SOCS1-STAT3 axis responses to oxidative stress in hepatocellular cancer cells. Am J Transl Res 13:12395-12409 (2021). PubMed: 34956461
- Lai S et al. Site-specific SUMOylation of viral polymerase processivity factor: a way of localizingtoND10 subnuclear domains for restricted and self-controlled reproduction of herpesvirus. Virulence 12:2883-2901 (2021). PubMed: 34747321
- Iwamoto H et al. Coffee diterpenes kahweol acetate and cafestol synergistically inhibit the proliferation and migration of prostate cancer cells. Prostate N/A:N/A (2018). PubMed: 30569541
- Ray S et al. Suppression of STAT3 NH2 -terminal domain chemosensitizes medulloblastoma cells by activation of protein inhibitor of activated STAT3 via de-repression by microRNA-21. Mol Carcinog 57:536-548 (2018). PubMed: 29280516
- Starsíchová A et al. TGF-beta1 suppresses IL-6-induced STAT3 activation through regulation of Jak2 expression in prostate epithelial cells. Cell Signal 22:1734-44 (2010). WB ; Human . PubMed: 20603212