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リコンビナントRabMAb

Anti-PIAS1 抗体 [EPR2580(2)] (ab109388)

Reviews (3) Submit a question References (17)
Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
  • Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
  • OI-RD Scanning - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
  • Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2580(2)] to PIAS1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-PIAS1 antibody [EPR2580(2)]
    PIAS1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2580(2)] to PIAS1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates. ICC/IF: HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109388の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
1/500.
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
特記事項
ICC/IF
1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.

  • 組織特異性

    Expressed in numerous tissues with highest level in testis.

  • パスウェイ

    Protein modification; protein sumoylation.

  • 配列類似性

    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.

  • ドメイン

    The LXXLL motif is a transcriptional coregulator signature.
    The SP-RING-type domain is required for promoting EKLF sumoylation.

  • 翻訳後修飾

    Sumoylated.
    Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.

  • 細胞内局在

    Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AR interacting protein antibody
    • DDXBP1 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
    • DEAD/H box binding protein 1 antibody
    • DEAD/H box-binding protein 1 antibody
    • E3 SUMO-protein ligase PIAS1 antibody
    • GBP antibody
    • Gu binding protein antibody
    • Gu-binding protein antibody
    • GU/RH-II antibody
    • Pias1 antibody
    • PIAS1_HUMAN antibody
    • Protein inhibitor of activated STAT protein 1 antibody
    • Protein inhibitor of activated STAT, 1 antibody
    • RNA helicase II binding protein antibody
    • RNA helicase II-binding protein antibody
    • Zinc finger, MIZ-type containing 3 antibody
    • ZMIZ3 antibody
    see all

画像

  • Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
    Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution

    All lanes : Recombinant Human PIAS1 protein (ab152888)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?



    Exposure time:

    Lane 1: 10 seconds.

    Lane 2: 1 minute.

     

    Blocking and dilution buffer: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PIAS1 with purified ab109388 at 1/500 dilution (4.6 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. 

  • Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : HEK-293 cell lysates
    Lane 4 : Daudi cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 72 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    OI-RD Scanning - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

参考文献 (17)

ab109388 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109388 は 17 報の論文で使用されています。

  • Tan D  et al. SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways. Nat Commun 14:3887 (2023). PubMed: 37393345
  • Lee YH  et al. A PIAS1 Protective Variant S510G Delays polyQ Disease Onset by Modifying Protein Homeostasis. Mov Disord 37:767-777 (2022). PubMed: 34951052
  • Wang G  et al. PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence. PLoS Pathog 18:e1010446 (2022). PubMed: 35377920
  • Huang M  et al. NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination. Aging (Albany NY) 14:3529-3539 (2022). PubMed: 35460552
  • Chen Q  et al. TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease. Front Physiol 12:709506 (2021). PubMed: 34434118
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-PIAS1 antibody [EPR2580(2)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEF)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
20 µg
Specification
MEF
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 07 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-PIAS1 antibody [EPR2580(2)]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton 5 minutes
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 26 2017

Western blot abreview for Anti-PIAS1 antibody [EPR2580(2)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 31 2017

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