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Epigenetics and Nuclear Signaling Nuclear Signaling Pathways Nuclear Receptors Co-activators/co-repressors
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Anti-PIAS1 抗体 (ab32219)

  • Datasheet
  • SDS
Reviews (6)Q&A (2)References (17)

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Promotion Information

Abpromise

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Western blot - Anti-PIAS1 antibody (ab32219)
  • Western blot - Anti-PIAS1 antibody (ab32219)
  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody (ab32219)
  • Western blot - Anti-PIAS1 antibody (ab32219)
  • Western blot - Anti-PIAS1 antibody (ab32219)

Key features and details

  • Rabbit polyclonal to PIAS1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PIAS1 antibody
    PIAS1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to PIAS1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Human, Recombinant fragment
    交差が予測される動物種: Rat, Chicken, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human PIAS1 aa 500-600.
    (Peptide available as ab32218)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

関連製品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32219の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
Use a concentration of 1 µg/ml.
WB (3)
1/500 - 1/2000. Detects a band of approximately 81 kDa (predicted molecular weight: 72 kDa).
特記事項
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/500 - 1/2000. Detects a band of approximately 81 kDa (predicted molecular weight: 72 kDa).

ターゲット情報

  • 機能

    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
  • 組織特異性

    Expressed in numerous tissues with highest level in testis.
  • パスウェイ

    Protein modification; protein sumoylation.
  • 配列類似性

    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • ドメイン

    The LXXLL motif is a transcriptional coregulator signature.
    The SP-RING-type domain is required for promoting EKLF sumoylation.
  • 翻訳後修飾

    Sumoylated.
    Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
  • 細胞内局在

    Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
  • Target information above from: UniProt accession O75925 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 427514 Chicken
    • Entrez Gene: 8554 Human
    • Entrez Gene: 56469 Mouse
    • Entrez Gene: 300772 Rat
    • Omim: 603566 Human
    • SwissProt: O75925 Human
    • SwissProt: O88907 Mouse
    • Unigene: 162458 Human
    • Unigene: 431253 Mouse
    • Unigene: 479179 Mouse
    see all
  • 別名

    • AR interacting protein antibody
    • DDXBP1 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
    • DEAD/H box binding protein 1 antibody
    • DEAD/H box-binding protein 1 antibody
    • E3 SUMO-protein ligase PIAS1 antibody
    • GBP antibody
    • Gu binding protein antibody
    • Gu-binding protein antibody
    • GU/RH-II antibody
    • Pias1 antibody
    • PIAS1_HUMAN antibody
    • Protein inhibitor of activated STAT protein 1 antibody
    • Protein inhibitor of activated STAT, 1 antibody
    • RNA helicase II binding protein antibody
    • RNA helicase II-binding protein antibody
    • Zinc finger, MIZ-type containing 3 antibody
    • ZMIZ3 antibody
    see all

画像

  • Western blot - Anti-PIAS1 antibody (ab32219)
    Western blot - Anti-PIAS1 antibody (ab32219)This image is courtesy of an Abreview submitted by Min young Kim
    All lanes : Anti-PIAS1 antibody (ab32219) at 1/2500 dilution (Incubation for 1 hour at 25°C.)

    Lane 1 : 293T cell lysate
    Lane 2 : MEL cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 71 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-PIAS1 antibody (ab32219)
    Western blot - Anti-PIAS1 antibody (ab32219)
    All lanes : Anti-PIAS1 antibody (ab32219) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate at 25 µg
    Lane 2 : HeLa whole cell extract with transfected PIAS1 at 25 µg
    Lane 3 : HeLa nuclear extract at 25 µg
    Lane 4 : T47D nuclear extract
    at 25 µg
    Lane 5 : HeLa whole cell lysate immunoprecipitated with ab32219
    Lane 6 : HeLa whole cell extract with transfected PIAS1 immunoprecipitated with ab32219
    Lane 7 : HeLa nuclear extract immunoprecipitated with ab32219
    Lane 8 : T47D nuclear extract immunoprecipitated with ab32219


    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 81 kDa why is the actual band size different from the predicted?
    Additional bands at: 110 kDa, 79 kDa (possible cleavage fragment), 79 kDa (possible cross reactivity). We are unsure as to the identity of these extra bands.



    ab32219 was used in western blot analysis using HeLa whole cell lysate, HeLa nuclear lysate and T47D nuclear lysate. It was unable to detect endogenous PIAS1 in these lysates. It was able to detect PIAS1 in cells that are overexpressing the protein, running at a molecular weight of approximately 81kDa, suggesting that the amount of endogenous PIAS1 in these cells is very low. When ab32219 was used to immunoprecipitate the PIAS1 from these extracts, the protein could then be detected by western blot in the overexpressing HeLa extract as well as the T47D nuclear extract.For immunoprecipitations, 25ug of extract was used in the IP, and 10% of the sample was run on the gel.
  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody (ab32219)
    Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody (ab32219)
    ICC/IF image of ab32219 stained MEF1 cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32219, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-PIAS1 antibody (ab32219)
    Western blot - Anti-PIAS1 antibody (ab32219)
    Anti-PIAS1 antibody (ab32219) at 1 µg/ml + PIAS1 - Recombinant Protein at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    ab32219 recognizes the full length tagged recombinant protein which has an expected molecular weight of 98 kDa.
  • Western blot - Anti-PIAS1 antibody (ab32219)
    Western blot - Anti-PIAS1 antibody (ab32219)
    Anti-PIAS1 antibody (ab32219) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 72 kDa
    Additional bands at: 180 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (17)

ab32219 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32219 は 17 報の論文で使用されています。

  • Chen H  et al. Mmu_circ_0000037 inhibits the progression of acute pancreatitis by miR-92a-3p/Pias1 axis. Immun Inflamm Dis 11:e819 (2023). PubMed: 37102653
  • Lee D  et al. ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination. Nucleic Acids Res 50:3922-3943 (2022). PubMed: 35253893
  • Lee YH  et al. A PIAS1 Protective Variant S510G Delays polyQ Disease Onset by Modifying Protein Homeostasis. Mov Disord 37:767-777 (2022). PubMed: 34951052
  • Lin HY  et al. SUMO E3 ligase PIAS1 is a potential biomarker indicating stress susceptibility. Psychoneuroendocrinology 120:104800 (2020). PubMed: 32688147
  • Wang Z  et al. Hypermethylation of miR-181b in monocytes is associated with coronary artery disease and promotes M1 polarized phenotype via PIAS1-KLF4 axis. Cardiovasc Diagn Ther 10:738-751 (2020). PubMed: 32968630
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-PIAS1 antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 27 2018

Western blot abreview for Anti-PIAS1 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (EC109)
Gel Running Conditions
Non-reduced Denaturing (10% SDS)
Loading amount
80 µg
Specification
EC109
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 31 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PIAS1 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human breast cancer section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Permeabilization
Yes - tween-20
Specification
human breast cancer section
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 17 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-PIAS1 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (erythroid cell)
Specification
erythroid cell
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 23 2013

Western blot abreview for Anti-PIAS1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (293T cell , MEL cell, K562 cell)
Specification
293T cell , MEL cell, K562 cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Min Young Kim

Verified customer

投稿 Jun 25 2013

ChIP abreview for Anti-PIAS1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - whole cell (LIVER)
Specification
LIVER
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37%FORMALDEHYDE
Detection step
Semiquantitative PCR
Positive control
pol11
Negative control
IgG
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Natarajan Balasubramaniyan

Verified customer

投稿 May 19 2012

Question

Dear Dr Keith Yes,I want use in the beginning ab32219 and ab32058 for the ChiP assay in human liver cell line.Based on the results I want use other antibody as well.I am going to order all the antibody now.Thanks. With regards Bala ________________________________________ From: technical@abcam.com [technical@abcam.com] Sent: Thursday, January 12, 2012 3:27 PM To: Balasubramaniyan, Natarajavelalar Subject: Reply from Abcam to your enquiry regarding ab32058, ab32219, ab75854, ab81371 [CCE3421816] [https://www.abcam.com/ps/cms/emails/CRM-Header.jpg] Dear Sir/Madam We have an answer to your inquiry: Thank you very much for your interest in ab32219, ab32058, ab81371, ab75854. To our knowledge, these products has not been tested in ChIP on human. Therefore, I can offer a discount off a future purchase if you buy now, test in ChIP on human and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test any or each of these products in ChIP on human. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering. 2. Purchase the antibody or antibodies either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Help us improve our service. Rate your experience with us today. Your original inquiry to Abcam: Will these work on ChIP as well? Best regards, Keith Keith Beadle Scientific Support Specialist Abcam Inc. www.abcam.com Abcam Customer Services and Scientific Support Team www.abcam.com/technical [CCE3421816] Discover more at abcam.com

Read More

Abcam community

Verified customer

Asked on Jan 13 2012

Answer

Ab32058 DISCOUNT CODE: Expiration date: Ab32058 DISCOUNT CODE: Expiration date: Ab81371 DISCOUNT CODE: Expiration date: 14 May 2012 Ab75854 DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and testthese productsinChIP. This code will give you 1 free PRIMARY ANTIBODYper codebefore the expiration date. To redeem this offer, please submit an Abreview foreach product in ChIPand include the associatedcode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Jan 13 2012

Question

Will these work on ChIP as well?

Read More

Abcam community

Verified customer

Asked on Jan 12 2012

Answer

Thank you very much for your interest in ab32219, ab32058, ab81371, ab75854. To our knowledge, these products has not been tested in ChIP on human. Therefore, I can offer a discount off a future purchase if you buy now, test inChIP on human and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and testany or each of these productsin ChIP on human. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering. 2. Purchasethe antibody or antibodieseither by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Jan 12 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
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