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AB307099

Anti-PI3-Kinase p170 抗体 [17/PI3-Kinase p170] - BSA and Azide Free

Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free

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Mouse Recombinant Monoclonal PI 3 Kinase Class 2A/Cpk antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

別名を表示する

Cpk, Pik3c2a, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, PI3K-C2-alpha, PtdIns-3-kinase C2 subunit alpha, Cpk-m, Phosphoinositide 3-kinase-C2-alpha, p170

1 Images
Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free (AB307099)
  • WB

Supplier Data

Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free (AB307099)

This data was developed using ab307098, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : liver, brain (PMID : 9337861). The expression molecular weight observed is consistent with what has been described in the literature (PMID : 8662856). Exposure times : Lane 1 : 59 seconds Lane 2-9 : 3 minutes

All lanes:

Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] (<a href='/products/primary-antibodies/pi3-kinase-p170-antibody-17-pi3-kinase-p170-ab307098'>ab307098</a>) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate

Lane 2:

Mouse testis tissue lysate

Lane 3:

Mouse pancreas tissue lysate

Lane 4:

Mouse liver tissue lysate

Lane 5:

Mouse brain tissue lysate

Lane 6:

Rat testis tissue lysate

Lane 7:

Rat brain tissue lysate

Lane 8:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lane 9:

C6 (rat glial tumor glial cell), whole cell lysate

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 210 kDa,190 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

17/PI3-Kinase p170

アイソタイプ

IgG1

キャリアフリー

Yes

交差種

Mouse, Rat

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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製品の詳細

ab307099 is the carrier-free version of ab307098.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: 100% PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PI 3 Kinase Class 2A also known as Cpk is a significant enzyme involved in the regulation of various cellular processes. The target weighing approximately 170 kDa acts as a lipid kinase that phosphorylates phosphatidylinositol converting PI to PI3P. Cpk expresses mainly in tissues like the liver muscle and heart where it contributes to cellular homeostasis and metabolic regulation.
Biological function summary

This enzyme family modulates intracellular signaling cascades influencing processes such as cell growth survival and metabolism. Cpk forms part of a larger complex that interacts with adaptor proteins facilitating its recruitment to cellular membranes. This interaction is important for its signaling activity and function in maintaining cellular equilibrium.

Pathways

PI 3 Kinase Class 2A plays an integral role in the insulin signaling pathway and the TOR signaling pathway. In the insulin pathway it collaborates with proteins like Akt to modulate glucose uptake and storage in cells. Similarly in the TOR pathway Cpk contributes to nutrient sensing and cell growth regulation interconnected with proteins such as mTOR affecting protein synthesis and autophagy.

Dysregulation of PI 3 Kinase Class 2A links to cancer and type 2 diabetes. Alterations in its activity may lead to abnormal cell proliferation and survival characteristics of cancerous transformations associated with proteins like PTEN. Meanwhile its role in glucose metabolism ties it to insulin resistance and diabetes pathogenesis where interactions with insulin receptor substrates become important for disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms : involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components (By similarity).
See full target information Pik3c2a
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組合せ製品

Cell Lines & Lysates

AB264943

Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell line

Sanger Sequencing - Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell line (AB264943)

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Cell Lines & Lysates

AB259040

Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell lysate

Sanger Sequencing - Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell lysate (AB259040)

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Primary Antibodies

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Primary Antibodies

AB278545

Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

20ul selling size|Recombinant

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

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