AB32569

Anti-PI3 Kinase p110 beta 抗体 [Y384]

Name: Anti-PI3 Kinase p110 beta 抗体 [Y384] (ab32569) | Abcam
Brand: Abcam Limited
SKU: AB32569
Rating: 5 (1 reviews)

Anti-PI3 Kinase p110 beta antibody [Y384]

RabMAb
Recombinant
KO Validated
20ul selling size
詳細を見る

(1 Review)

(18 Publications)

Rabbit Recombinant Monoclonal PI3 Kinase p110 beta antibody. Suitable for IP, WB and reacts with Human samples. Cited in 18 publications.

別名を表示する

PIK3C1, PIK3CB, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Serine/threonine protein kinase PIK3CB, PtdIns-3-kinase subunit p110-beta, p110beta

3 Images

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Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

All lanes:

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) at 1/500 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 123 kDa

Observed band size: 110 kDa

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Lab

Immunoprecipitation - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

PI3 Kinase p110 beta was immunoprecipitated from 0.35 mg Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 μg with ab32569 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 μg

Lane 2 : ab32569 IP in Jurkat whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab32569 in Jurkat whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)

Predicted band size: 123 kDa

Observed band size: 110 kDa

false

Lab

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : PI3 Kinase p110 beta knockout HAP1 cell lysate (40 μg)
Lane 3 : Jurkat cell lysate (40 μg)
Lane 4 : K562 cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab32569 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32569 was shown to recognize PI3 Kinase p110 beta when PI3 Kinase p110 beta knockout samples were used, along with additional cross-reactive bands. Wild-type and PI3 Kinase p110 beta knockout samples were subjected to SDS-PAGE. ab32569 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)

Predicted band size: 123 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

Y384

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab32569 recognises PI3-Kinases (PI3-Ks).

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Reactivity data

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phosphatidylinositol 3-kinase p110 beta (PI3 kinase p110β) also known as PI3Kβ is an enzyme that belongs to the class I PI3-kinases. It functions as a catalytic subunit in the PI3K complex. The PI3 kinase p110β has a molecular mass of approximately 122 kDa. This protein is expressed in various tissues with notable presence in heart and skeletal muscles. The enzyme is responsible for phosphorylating PIP2 to generate PIP3 an essential step in signaling pathways that regulate cell growth and survival.

Biological function summary

PI3 kinase p110β plays a role in the regulation of cellular processes such as proliferation migration and metabolism. It is a component of the PI3K complex comprised of regulatory and catalytic subunits which form functional homodimers or heterodimers. This complex is activated by various receptors including G-protein coupled receptors (GPCRs) and tyrosine kinase receptors. By converting PIP2 to PIP3 it aids in the recruitment and activation of downstream signaling proteins like AKT.

Pathways

PI3 kinase p110β is integral in the PI3K/AKT signaling pathway and also contributes to the regulation of the mTOR pathway. These pathways are critical for controlling cellular responses to growth signals and maintaining homeostasis. Association with proteins like PTEN which negatively regulates the PI3K/AKT pathway highlights its importance in maintaining cellular balance. PI3 kinase p110β also interacts with other PI3 proteins like p110α showing the intricate network of signaling it participates in.

PI3 kinase p110β has been implicated in cancer and cardiovascular diseases. Its overactivation leads to unchecked cellular proliferation contributing significantly to oncogenesis. Alterations in the PI3K/AKT/mTOR pathway are common in breast cancer highlighting its interaction with proteins such as ERBB2. In cardiovascular disorders PI3 kinase p110β influences heart development and function where it can impact the action of related proteins like PI3 kinase p110α. The study of PI3 kinase p110β in these diseases offers potential for targeted therapies and improved understanding of its pathological roles.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed : 12502714).

See full target information PIK3CB

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed34830882

2021

Effects of Aerobic Exercise Training on MyomiRs Expression in Cachectic and Non-Cachectic Cancer Mice.

Applications

Unspecified application

Species

Unspecified reactive species

João Lucas Penteado Gomes,Gabriel Cardial Tobias,Tiago Fernandes,André Casanova Silveira,Carlos Eduardo Negrão,Roger Chammas,Patrícia Chakur Brum,Edilamar Menezes Oliveira

Frontiers in cell and developmental biology 9:720925 PubMed34790657

2021

The RNA Methyltransferase METTL3 Promotes Endothelial Progenitor Cell Angiogenesis in Mandibular Distraction Osteogenesis via the PI3K/AKT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong Jiang,Peiqi Zhu,Fangfang Huang,Zhenchen Zhao,Tao Zhang,Xiaoning An,Fengchun Liao,Lina Guo,Yan Liu,Nuo Zhou,Xuanping Huang

Scientific reports 11:15761 PubMed34344927

2021

The COL-4A1 polypeptide destroy endothelial cells through the TGF-β/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Li,Zhonghui Ling,Kaipeng Xie,Yixiao Wang,Zhijing Miao,Xiaohong Ji,Jingyun Li,Wenwen Hou,Qiuqin Tang,Xiaojie Yuan,Nan Li,Chanjuan Li,Hongjuan Ding

Oncology reports 44:1333-1342 PubMed32945504

2020

Ginsenoside Rg3 enhances the anticancer effect of 5‑FU in colon cancer cells via the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shunzhong Hong,Wenjie Cai,Zicheng Huang,Yubin Wang,Xifeng Mi,Yisen Huang,Zhijin Lin,Xiangbo Chen

Journal of immunology (Baltimore, Md. : 1950) 203:137-147 PubMed31092639

2019

Effects of PI3K Inhibition on Afucosylated Antibody-Driven FcγRIIIa Events and Phospho-S6 Activity in NK Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Romeo,Sarah Gierke,Kyle A Edgar,Scot D Liu

Oncology letters 15:1829-1838 PubMed29399196

2018

Microarray pathway analysis indicated that mitogen-activated protein kinase/extracellular signal-regulated kinase and insulin growth factor 1 signaling pathways were inhibited by small interfering RNA against AT-rich interactive domain 1A in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yang,Wei Bao,Zhengyu Sang,Yongbing Yang,Meng Lu,Xiaowei Xi

Medical science monitor : international medical jo 24:623-635 PubMed29384143

2018

Expression and Comparison of Cbl-b in Lung Squamous Cell Carcinoma and Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Li,Hongliang Liu,Zhiqiang Zhang,Xiaodong Lv,Huijuan Wang,Jie Ma,Zhiyong Ma,Xiujuan Qu,Yue-E Teng

Experimental and therapeutic medicine 14:1967-1974 PubMed28962111

2017

MicroRNA-138 suppresses cell proliferation in laryngeal squamous cell carcinoma via inhibiting EZH2 and PI3K/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fengzhi Si,Jie Sun,Chunli Wang

Oncology reports 35:3648-58 PubMed27035545

2016

Diallyl trisulfide induces osteosarcoma cell apoptosis through reactive oxygen species-mediated downregulation of the PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongliang Wang,Na Sun,Xin Li,Ka Li,Jiguang Tian,Jianmin Li

The Journal of biological chemistry 289:35953-68 PubMed25384981

2014

Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of ΔF508-cystic fibrosis transmembrane conductance regulator (ΔF508-CFTR).

Applications

Unspecified application

Species

Unspecified reactive species

Hung Caohuy,Qingfeng Yang,Yvonne Eudy,Thien-An Ha,Andrew E Xu,Matthew Glover,Raymond A Frizzell,Catherine Jozwik,Harvey B Pollard

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PI3 Kinase p110 beta 抗体 [Y384]

Anti-PI3 Kinase p110 beta antibody [Y384]

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

Immunoprecipitation - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (18)

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