Key features and details
- Mouse monoclonal [T15] to PI 3 Kinase p85 beta
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
製品名Anti-PI 3 Kinase p85 beta antibody [T15]
PI 3 Kinase p85 beta 一次抗体 製品一覧
製品の詳細Mouse monoclonal [T15] to PI 3 Kinase p85 beta
特異性T15 recognises the p85 beta subunit of bovine PI 3 kinase. It does not cross react with the p85 alpha subunit.
アプリケーション適用あり: WBmore details
交差が予測される動物種: Mouse, Rat
Balculovirus expressed (Recombinant) bovine P85 beta subunit of PI 3 kinase.
- WB: HEK293T cell lysate; Lysates of SF9 cells infected with baculovirus expressing p85 beta.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
バッファーPreservative: 0.09% Sodium azide
Concentration information loading...
精製度Protein G purified
特記事項（精製）This antibody is purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
KO cell lines
KO cell lysates
The Abpromise guarantee
1/1000 - 1/5000. Predicted molecular weight: 82 kDa.
1/1000 - 1/5000. Predicted molecular weight: 82 kDa.
機能Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
関連疾患Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
配列類似性Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
ドメインThe SH2 2 domain is required for interaction with FBXL2 and PTPN13.
翻訳後修飾Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
- Information by UniProt
- p85 antibody
- p85 beta antibody
- P85B antibody
All lanes : Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PIK3R2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Lanes 1- 2: Merged signal (red and green). Green - ab28356 observed at 85 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab28356 was shown to react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type HEK-293T and PIK3R2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab28356 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western Blotting of HEK293 and HepG2 cell lysates with Mouse anti Bovine PI-3 kinase p85 subunit beta (ab28356), recombinant p85ß is included as a positive control.
ab28356 は 13 報の論文で使用されています。
- Song KW et al. RTK-Dependent Inducible Degradation of Mutant PI3Kα Drives GDC-0077 (Inavolisib) Efficacy. Cancer Discov 12:204-219 (2022). PubMed: 34544753
- Zheng D et al. Long Non-coding RNA Gas5 Is Associated With Preeclampsia and Regulates Biological Behaviors of Trophoblast via MicroRNA-21. Front Genet 11:188 (2020). PubMed: 32194641
- Pedini F et al. Joint action of miR-126 and MAPK/PI3K inhibitors against metastatic melanoma. Mol Oncol 13:1836-1854 (2019). PubMed: 31115969
- Yang MH et al. Reduction of aluminum ion neurotoxicity through a small peptide application - NAP treatment of Alzheimer's disease. J Food Drug Anal 27:551-564 (2019). PubMed: 30987727
- Dai K et al. Kbtbd2 inhibits the cytotoxic activity of immortalized NK cells through down-regulating mTOR signaling in a mouse hepatocellular carcinoma model. Eur J Immunol 48:683-695 (2018). PubMed: 29331106