AB74136

Anti-PI 3 Kinase p85 alpha + Gamma 抗体

Name: Anti-PI 3 Kinase p85 alpha + Gamma 抗体 (ab74136) | Abcam
Brand: Abcam Limited
SKU: AB74136
Price: 76000 JPY
Availability: InStock
Rating: 3 (2 reviews)

Anti-PI 3 Kinase p85 alpha + Gamma antibody

(2 Reviews)

(12 Publications)

Rabbit Polyclonal PI 3 Kinase p85 alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with African green monkey, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human PIK3R1.

別名を表示する

GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

Immunofluorescence analysis of HeLa cells using ab74136, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab74136, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

ICC/IF

AbReview17333****

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

ab74136 at 1/200 dilution staining PI 3 Kinase p85 alpha + Gamma in mouse embryonic stem cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized in 1 : 1 methanol, acetone and blocked in 1% serum for 30 minutes at 25°C. The primary antibody was used at 1/100 dilution in Goat block dillution buffer and incubated with sample at 25°C for 1 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG (H+L) was used as secondary at 1/500 dilution.

Unknown

Western blot - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

All lanes:

Western blot - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136) at 1/500 dilution

Lane 1:

Extracts from COS7 cells, treated with H2O2 (100µM, 30mins) at 5 µg

Lane 2:

Extracts from COS7 cells, treated with H2O2 (100µM, 30mins) at 5 µg with immunising peptide

Predicted band size: 84 kDa

Observed band size: 70 kDa,84 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, African green monkey

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human PIK3R1. The exact immunogen used to generate this antibody is proprietary information.

P27986

特異性

PI3 kinase p85 alpha and gamma.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The PI 3 Kinase p85 alpha + Gamma also known as PI3K is a regulatory subunit of the class IA phosphoinositide 3-kinases. PI3K is comprised of the p85 alpha (approximately 83 kDa) and p110 gamma subunits among others and plays an important role in cell signaling. The p85 alpha subunit is primarily expressed in immune cells liver and muscles while the p110 gamma is mostly found in leukocytes. This kinase is deeply involved in a variety of cellular processes like cell growth proliferation differentiation motility and survival.

Biological function summary

The PI 3 Kinase p85 alpha + Gamma is an important regulator in signal transduction pathways and forms a heterodimer complex with p110 catalytic subunits. This kinase complex is activated by various growth factors and hormones through receptor tyrosine kinases. Upon activation PI3K phosphorylates phosphatidylinositol (45)-bisphosphate (PIP2) to produce phosphatidylinositol (345)-trisphosphate (PIP3) an essential second messenger that recruits downstream signaling proteins like AKT to the plasma membrane. This signal cascade regulates numerous cellular functions affecting homeostasis and adaptive responses.

Pathways

The PI 3 Kinase p85 alpha + Gamma plays a significant role in the PI3K/AKT/mTOR and MAPK signaling pathways. These pathways are essential in controlling cell metabolism survival and growth. Within these cascades PI3K phosphorylates PIP2 to PIP3 creating a docking site for AKT which subsequently becomes activated and relays signals to downstream effectors including mTOR and GSK-3β. The proper functioning of these pathways is necessary for maintaining cellular equilibrium and their dysregulation might lead to pathological conditions.

Aberrant PI 3 Kinase p85 alpha + Gamma activity is implicated in cancer and immune disorders. In particular its malfunction is notable in various cancers including breast and prostate cancer where mutations can lead to constitutive activation of PI3K signaling promoting tumorigenic processes. Additionally mutations or altered expression of PI3K can contribute to immune-related conditions by affecting immune cell function and survival. Interactions between PI3K and other proteins such as PTEN a tumor suppressor represent key nodes in understanding these disease mechanisms and offer potential therapeutic targets.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed : 17626883, PubMed : 19805105, PubMed : 7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed : 20348923).

See full target information PIK3R1

追加のターゲット

PIK3R3

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medic 2020:1403572 PubMed32802113

2020

In Vitro Evaluation of the Neuroprotective Effect of Saponins by Activating the EGFR/PI3K/AKT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Wu,Tiantian Yang,Kai Cen,Yihuai Zou,Xiaowei Shi,Dongrui Zhou,Yonghong Gao,Limin Chai,Yizhou Zhao,Yikun Sun,Lingqun Zhu

Evidence-based complementary and alternative medic 2020:4527283 PubMed32774416

2020

Shenzhiling Oral Liquid Protects STZ-Injured Oligodendrocyte through PI3K/Akt-mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhong Liu,Gaofeng Qin,Lulu Mana,Shuaiyang Huang,Yahan Wang,Pengwen Wang

Bioscience reports 40: PubMed31904088

2020

Dynamic characterization of intestinal metaplasia in the gastric corpus mucosa of Atp4a-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Liang-Jun Yang,Yuan-Liang Liu,Dong-Sheng Yuan,Zi-Ming Zhao,Qi Wang,Yan Yan,Hua-Feng Pan

Food & function 10:2161-2175 PubMed30938722

2019

Inhibiting the PI3K/AKT/NF-κB signal pathway with nobiletin for attenuating the development of osteoarthritis: in vitro and in vivo studies.

Applications

Unspecified application

Species

Unspecified reactive species

Linzhen Xie,Huanguang Xie,Chunhui Chen,Zhenyu Tao,Chuanxu Zhang,Leyi Cai

Rejuvenation research 22:218-229 PubMed30215298

2018

Aloe-Emodin Ameliorates Renal Fibrosis Via Inhibiting PI3K/Akt/mTOR Signaling Pathway and .

Applications

Unspecified application

Species

Unspecified reactive species

Fang Dou,YueTong Liu,Limin Liu,Jingwen Wang,Ting Sun,Fei Mu,Qiyan Guo,Chao Guo,Na Jia,Wenxin Liu,Yi Ding,Aidong Wen

Molecular medicine reports 16:7706-7714 PubMed28944880

2017

Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Song,Hongbo Ci,Guanglei Tian,Yanjun Zhang,Xiaohu Ge

International journal of immunopathology and pharm 30:25-43 PubMed28124574

2017

Curcumin regulates insulin pathways and glucose metabolism in the brains of APPswe/PS1dE9 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pengwen Wang,Caixin Su,Huili Feng,Xiaopei Chen,Yunfang Dong,Yingxue Rao,Ying Ren,Jinduo Yang,Jing Shi,Jinzhou Tian,Shucui Jiang

International journal of immunopathology and pharm 29:734-741 PubMed27466310

2016

Curcumin ameliorates insulin signalling pathway in brain of Alzheimer's disease transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Li Feng,Hui-Zi Dang,Hui Fan,Xiao-Pei Chen,Ying-Xue Rao,Ying Ren,Jin-Duo Yang,Jing Shi,Peng-Wen Wang,Jin-Zhou Tian

Biochemistry and biophysics reports 5:313-327 PubMed28955838

2016

Mangiferin attenuates oxidative stress induced renal cell damage through activation of PI3K induced Akt and Nrf-2 mediated signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sukanya Saha,Pritam Sadhukhan,Krishnendu Sinha,Namrata Agarwal,Parames C Sil

British journal of cancer 110:1968-76 PubMed24595002

2014

Clinical significance of p95HER2 overexpression, PTEN loss and PI3K expression in p185HER2-positive metastatic breast cancer patients treated with trastuzumab-based therapies.

Applications

Unspecified application

Species

Human

D Tural,S Serdengecti,F Demirelli,T Öztürk,S İlvan,H Turna,M Özgüroglu,E Büyükünal

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-PI 3 Kinase p85 alpha + Gamma 抗体

Anti-PI 3 Kinase p85 alpha + Gamma antibody

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

Western blot - Anti-PI 3 Kinase p85 alpha + Gamma antibody (AB74136)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (12)

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