Anti-PI 3 Kinase p85 alpha 抗体 [EP380Y] (ab40755)
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)](https://www.abcam.co.jp/ps/products/40/ab40755/Images/ab40755-255514-anti-pi-3-kinase-p85-alpha-antibody-ep380y-western-blot.jpg "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP380Y] to PI 3 Kinase p85 alpha
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PI 3 Kinase p85 alpha antibody [EP380Y]
PI 3 Kinase p85 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP380Y] to PI 3 Kinase p85 alpha -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human PI 3 Kinase p85 alpha aa 700-800 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- A431, MCF7 or Raji cells lysate, HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP380Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40755の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/1000 - 1/2000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
ターゲット情報
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機能
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. -
組織特異性
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). -
配列類似性
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef. -
翻訳後修飾
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Dephosphorylated by PTPRJ. - Information by UniProt
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参照データベース
- Entrez Gene: 5295 Human
- Entrez Gene: 18708 Mouse
- Entrez Gene: 25513 Rat
- Omim: 171833 Human
- SwissProt: P27986 Human
- SwissProt: P26450 Mouse
- SwissProt: Q63787 Rat
- Unigene: 132225 Human
see all -
別名
- GRB1 antibody
- p85 alpha antibody
- p85 antibody
see all
画像
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Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PI 3 Kinase p85 alpha knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: THP1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab40755 observed at 90 kDa. Red - loading control, ab181602, observed at 37 kDa.ab40755 was shown to recognize PI 3 Kinase p85 alpha in wild-type HAP1 cells along with additional cross-reactive bands. No Band was observed when PI 3 Kinase p85 alpha knockout samples were examined. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. ab40755 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)](https://www.abcam.co.jp/ps/products/40/ab40755/Images/ab40755-53859-anti-pi-3-kinase-p85-alpha-antibody-ep380y-western-blot.jpg)
Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)All lanes : Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755) at 1/2000 dilution
Lane 1 : A431 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa -
![Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)](https://www.abcam.co.jp/ps/products/40/ab40755/Images/ab40755-5-benefits-of-recombinant-antibodies.png)
Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (40)
ab40755 は 40 報の論文で使用されています。
- Li N et al. Streptococcus pneumoniae promotes lung cancer development and progression. iScience 26:105923 (2023). PubMed: 36685035
- Su X et al. Astragaloside IV attenuates high glucose-induced NF-κB-mediated inflammation through activation of PI3K/AKT-ERK-dependent Nrf2/ARE signaling pathway in glomerular mesangial cells. Phytother Res 37:4133-4148 (2023). PubMed: 37189016
- Yoo DY et al. Isoflavone-Enriched Soybean Leaves (Glycine Max) Alleviate Cognitive Impairment Induced by Ovariectomy and Modulate PI3K/Akt Signaling in the Hippocampus of C57BL6 Mice. Nutrients 14:N/A (2022). PubMed: 36432439
- Wang CY et al. Involvement of FoxO1, Sp1, and Nrf2 in Upregulation of Negative Regulator of ROS by 15d-PGJ2 Attenuates H2O2-Induced IL-6 Expression in Rat Brain Astrocytes. Neurotox Res 40:154-172 (2022). PubMed: 34997457
- Ding J et al. LPCAT1 promotes gefitinib resistance via upregulation of the EGFR/PI3K/AKT signaling pathway in lung adenocarcinoma. J Cancer 13:1837-1847 (2022). PubMed: 35399731