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リコンビナントRabMAb

Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma 抗体 [EPR25156-60] - BSA and Azide free (ab302959)

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Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
  • Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25156-60] to PI 3 Kinase catalytic subunit gamma/PI3K-gamma - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free
    PI 3 Kinase catalytic subunit gamma/PI3K-gamma 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25156-60] to PI 3 Kinase catalytic subunit gamma/PI3K-gamma - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, IPmore details
    適用なし: Flow Cyt (Intra) or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: K562, Jurkat, PC-12, RAW264.7 whole cell lysates; mouse thymus and spleen tissue lysates. IHC-P: Human tonsil, spleen, and breast carcinoma; mouse and rat spleen FFPE tissue sections; HEK-293T cells transfected with a PIK3CG expression vector containing a his tag (cell pellet). IP: Jurkat and RAW264.7 whole cell lysates.

  • 特記事項

    ab302959 is the carrier-free version of ab302958.

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302959の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 127 kDa (predicted molecular weight: 126 kDa).
IP
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 127 kDa (predicted molecular weight: 126 kDa).
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

ターゲット情報

  • 機能

    3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.

  • 組織特異性

    Pancreas, skeletal muscle, liver and heart.

  • パスウェイ

    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.

  • 配列類似性

    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 1 phosphatidylinositol 3 kinase antibody
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
    • 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
    • p110 gamma antibody
    • p110gamma antibody
    • p120 PI3K antibody
    • p120-PI3K antibody
    • Phosphatidylinositol 3 kinase catalytic 110 kD gamma antibody
    • Phosphatidylinositol 3 kinase gamma, p110 gamma antibody
    • Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic gamma polypeptide antibody
    • Phosphoinositide 3 kinase gamma catalytic subunit antibody
    • PI 3 Kinase catalytic subunit gamma antibody
    • PI3 kinase p110 subunit gamma antibody
    • PI3-kinase subunit gamma antibody
    • PI3CG antibody
    • PI3K antibody
    • PI3K-gamma antibody
    • PI3Kgamma antibody
    • PIK3 antibody
    • Pik3cg antibody
    • PK3CG_HUMAN antibody
    • PtdIns-3-kinase subunit gamma antibody
    • PtdIns-3-kinase subunit p110-gamma antibody
    • Serine/threonine protein kinase PIK3CG antibody
    see all

画像

  • Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (ab302958) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 126 kDa
    Observed band size: 127 kDa why is the actual band size different from the predicted?



    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    Negative control: SW480 (PMID: 12473596), A549 (PMID: 27030971)

    Exposure time:

    Lanes 1-5: 15 seconds
    Lane 6: 180 seconds

  • Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] (ab302958) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse thymus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 126 kDa
    Observed band size: 127 kDa why is the actual band size different from the predicted?



    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lanes 1-2: 3.25 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 dilution (5.02 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab302958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 dilution (5.02 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab302958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 dilution (5.02 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human breast carcinoma. The section was incubated with ab302958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 dilution (5.02 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse spleen. The section was incubated with ab302958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/100 dilution (5.02 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive staining on rat spleen. The section was incubated with ab302958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cell pellet labeling PI 3 Kinase catalytic subunit gamma/PI3K-gamma with ab302958 at 1/2000 dilution (0.251 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive staining on (A) HEK-293T cells transfected with a PIK3CG expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab302958 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

  • Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    PI 3 Kinase catalytic subunit gamma/PI3K-gamma was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte), whole cell lysate 10 µg with ab302958 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302958 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate 10 µg

    Lane 2: ab302958 IP in Jurkat whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302958 in Jurkat whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

     

  • Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

    This data was developed using ab302958, the same antibody clone in a different buffer formulation.

    PI 3 Kinase catalytic subunit gamma/PI3K-gamma was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate 10 µg with ab302958 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302958 at dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate 10 µg

    Lane 2: ab302958 IP in RAW264.7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302958 in RAW264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

     

  • Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)
    Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [EPR25156-60] - BSA and Azide free (ab302959)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab302959 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302959 は論文での使用が確認できていません。

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