Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma 抗体 (ab154598)
Key features and details
- Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody
PI 3 Kinase catalytic subunit gamma/PI3K-gamma 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human PI 3 Kinase catalytic subunit gamma/PI3K-gamma aa 1041-1102 (C terminal).
Database link: P48736 -
ポジティブ･コントロール
- WB: Transfected HEK-293T, Neuro 2A, RAW264.7, C8D30 and PC-12 cell lysate; IHC-P: Human breast cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154598の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 126 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 126 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ターゲット情報
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機能
3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production. -
組織特異性
Pancreas, skeletal muscle, liver and heart. -
パスウェイ
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. -
配列類似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. - Information by UniProt
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参照データベース
- Entrez Gene: 5294 Human
- Entrez Gene: 30955 Mouse
- Entrez Gene: 298947 Rat
- GenBank: AF327656 Human
- Omim: 601232 Human
- SwissProt: P48736 Human
- SwissProt: Q9JHG7 Mouse
- Unigene: 32942 Human
see all -
別名
- 1 phosphatidylinositol 3 kinase antibody
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
- 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining PI 3 Kinase catalytic subunit gamma with ab154598 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PI3K-gamma-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 126 kDa
7.5% SDS PAGE -
Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Predicted band size: 126 kDa7.5% SDS-PAGE
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Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 126 kDa5% SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab154598 は 36 報の論文で使用されています。
- Yu Q et al. Circular RNA-0072309 has antitumor influences in Hep3B cell line by targeting microRNA-665. Biofactors 49:79-89 (2023). PubMed: 32048412
- Gong L et al. MiRNA-106a-5p Promotes Laryngeal Carcinoma Proliferation and Migration Through PI3K/AKT/m-TOR Pathway by AKTIP. Iran J Biotechnol 21:e3339 (2023). PubMed: 36811106
- Lu JH et al. Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat. Int J Mol Sci 24:N/A (2023). PubMed: 37175945
- Meng P et al. A whole transcriptome profiling analysis for antidepressant mechanism of Xiaoyaosan mediated synapse loss via BDNF/trkB/PI3K signal axis in CUMS rats. BMC Complement Med Ther 23:198 (2023). PubMed: 37322430
- Geng G et al. S100A16 cooperates with DEPDC1 to promote the progression and angiogenesis of nephroblastoma through PI3K/Akt/mTOR pathway. Pol J Pathol 74:182-193 (2023). PubMed: 37955537