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AB227719

Anti-PHOX2B 抗体 - N-terminal

Anti-PHOX2B antibody - N-terminal

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(3 Publications)

Rabbit Polyclonal PHOX2B antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PHOX2B.

別名を表示する

PMX2B, PHOX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox, PHOX2B homeodomain protein, Paired-like homeobox 2B, NBPhox

2 Images
Western blot - Anti-PHOX2B antibody - N-terminal (AB227719)
  • WB

Supplier Data

Western blot - Anti-PHOX2B antibody - N-terminal (AB227719)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution

Lane 1:

IMR32 (human neuroblast cell line) whole cell extract at 30 µg

Lane 2:

SK-N-AS (human neuroblastoma cell line) whole cell extract at 30 µg

Predicted band size: 32 kDa

false

Western blot - Anti-PHOX2B antibody - N-terminal (AB227719)
  • WB

Supplier Data

Western blot - Anti-PHOX2B antibody - N-terminal (AB227719)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution

All lanes:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract at 30 µg

Predicted band size: 32 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human PHOX2B. The exact immunogen used to generate this antibody is proprietary information.

Q99453

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代替製品

Primary Antibodies

AB210658

Alexa Fluor® 594 Anti-PHOX2B antibody [EPR14423] - C-terminal

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-PHOX2B antibody [EPR14423] - C-terminal (AB210658)

  • 0
  • 0
Primary Antibodies

AB210459

Alexa Fluor® 488 Anti-PHOX2B antibody [EPR14423] - C-terminal

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PHOX2B antibody [EPR14423] - C-terminal (AB210459)

  • 0
  • 0
Primary Antibodies

AB216456

Anti-PHOX2B antibody [EPR14423] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free (AB216456)

  • 0
  • 0
Primary Antibodies

AB183741

Anti-PHOX2B antibody [EPR14423] - C-terminal

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (AB183741)

  • 5
  • 5

組合せ製品

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB211327

Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840]

BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (AB211327)

  • 4
  • 3
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHOX2B also known as Paired-like Homeobox 2B is a transcription factor that plays a significant role in neural development. It is encoded by the PHOX2B gene and has a molecular weight of approximately 31 kDa. This protein is highly expressed in the central and peripheral nervous systems where it influences the differentiation and function of neural crest-derived cells. PHOX2B is particularly active in the brainstem regions that control vital autonomic functions.
Biological function summary

PHOX2B influences the development of noradrenergic neurons and parasympathetic ganglia. It often functions as part of a transcription factor complex interacting with other proteins to drive the expression of genes required for the autonomic nervous system. The control PHOX2B exerts over neuronal differentiation makes it essential for maintaining the proper functioning of neurons responsible for involuntary bodily processes.

Pathways

PHOX2B is integral to the development and maintenance of the autonomic nervous and respiratory systems. It interacts closely with RET a receptor tyrosine kinase within the neurotrophic signaling pathways that regulate neuronal growth and survival. Additionally the protein plays a role in the endothelin signaling pathway further demonstrating its importance in neurodevelopment.

Alterations in PHOX2B have been linked to disorders such as congenital central hypoventilation syndrome (CCHS) and neuroblastoma. CCHS results from mutations in the PHOX2B gene affecting respiratory control. In the context of cancer PHOX2B neuroblastoma pertains to its potential role in tumor formation alongside MYCN amplification which is a common alteration in neuroblastoma. Understanding PHOX2B’s interactions provides insights into its potential as a target for therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
See full target information PHOX2B
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114134 PubMed38662542

2024

The MYCN 5' UTR as a therapeutic target in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marina P Volegova,Lauren E Brown,Ushashi Banerjee,Ruben Dries,Bandana Sharma,Alyssa Kennedy,John A Porco,Rani E George

Oxidative medicine and cellular longevity 2023:3308725 PubMed36785792

2023

MBNL1-AS1 Promotes Hypoxia-Induced Myocardial Infarction via the miR-132-3p/RAB14/CAMTA1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbing Li,Min Zong,Xiaonan Guan,Xuejiao Wu,Guiling Ma,Yu Wei,Zhi Li

Molecular therapy oncolytics 23:181-191 PubMed34729395

2021

Therapeutically targeting oncogenic CRCs facilitates induced differentiation of NB by RA and the BET bromodomain inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Satyanarayana Alleboina,Nour Aljouda,Mellessa Miller,Kevin W Freeman
View all publications
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