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リコンビナントRabMAb

Anti-PHOX2B 抗体 [EPR14423] - C-terminal (ab183741)

Reviews (4) Submit a question References (14)
Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
  • Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
  • Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14423] to PHOX2B - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-PHOX2B antibody [EPR14423] - C-terminal
    PHOX2B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14423] to PHOX2B - C-terminal

  • 由来種

    Rabbit

  • 特異性

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Neuro-2a and SH-SY5Y cell lysate. IHC-P: Human neuroblastoma tissue. ICC/IF: HeLa, Neuro-2a and SH-SY5Y cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183741の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P (2)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

ターゲット情報

  • 機能

    Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.

  • 組織特異性

    Expressed in neuroblastoma, brain and adrenal gland.

  • 関連疾患

    Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
    Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2) [MIM:613013]. A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.

  • 配列類似性

    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • NBLST2 antibody
      • NBPhox antibody
      • Neuroblastoma paired type homeobox protein antibody
      • Neuroblastoma Phox antibody
      • Paired like homeobox 2b antibody
      • Paired mesoderm homeobox 2b antibody
      • Paired mesoderm homeobox protein 2B antibody
      • Paired-like homeobox 2B antibody
      • PHOX 2B antibody
      • PHOX 2B homeodomain protein antibody
      • PHOX2B antibody
      • PHOX2B homeodomain protein antibody
      • PHX2B_HUMAN antibody
      • PMX 2B antibody
      • PMX2B antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
      Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cell line labelling PHOX2B with ab183741 at 1/100 (10 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

      Confocal image showing nuclear staining in Neuro-2a cell line.

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

      Immunohistochemical analysis of paraffin-embedded Human neuroblastoma tissue labeling PHOX2B  with ab183741 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
      Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
      All lanes : Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741) at 1/1000 dilution

      Lane 1 : Neuro-2a cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 32 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
      Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

      Immunofluorescence analysis of acetone-fixed HeLa cells labeling PHOX2B  with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
      Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

      Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling PHOX2B with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

    • Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
      Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    参考文献 (14)

    ab183741 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab183741 は 14 報の論文で使用されています。

    • Yarmarkovich M  et al. Targeting of intracellular oncoproteins with peptide-centric CARs. Nature 623:820-827 (2023). PubMed: 37938771
    • Sever RE  et al. Survival following complete resection of neuroblastoma in novel orthotopic rat xenograft model. Sci Rep 13:20214 (2023). PubMed: 37980388
    • Loh AHP  et al. Pro-metastatic and mesenchymal gene expression signatures characterize circulating tumor cells of neuroblastoma patients with bone marrow metastases and relapse. Front Oncol 12:939460 (2022). PubMed: 36176417
    • Gifford AJ  et al. A Primer for Assessing the Pathology in Mouse Models of Neuroblastoma. Curr Protoc 1:e310 (2021). PubMed: 34826366
    • Le TL  et al. Dysregulation of the NRG1/ERBB pathway causes a developmental disorder with gastrointestinal dysmotility in humans. J Clin Invest 131:N/A (2021). PubMed: 33497358
    View all Publications for this product

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-PHOX2B antibody [EPR14423] - C-terminal

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human embryo pelvis)
    Permeabilization
    No
    Specification
    Human embryo pelvis
    Blocking step
    Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Alain Chedotal

    Verified customer

    投稿 Jul 23 2024

    Immunohistochemistry (Frozen sections) abreview for Anti-PHOX2B antibody [EPR14423] - C-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Nodose Ganglia)
    Permeabilization
    Yes - 0.1% Triton x-100
    Specification
    Nodose Ganglia
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Saher Chaudry

    Verified customer

    投稿 Feb 08 2024

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PHOX2B antibody [EPR14423] - C-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (neuroblastoma)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    neuroblastoma
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 07 2023

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PHOX2B antibody [EPR14423] - C-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver bx - neuroblastoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
    Permeabilization
    No
    Specification
    Liver bx - neuroblastoma
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 29 2015

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