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AB155084

Anti-PHOX2A 抗体 [EPR9071]

Anti-PHOX2A antibody [EPR9071]

5

(4 Reviews)

|

(4 Publications)

Rabbit Recombinant Monoclonal PHOX2A antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.

別名を表示する

ARIX, PMX2A, PHOX2A, Paired mesoderm homeobox protein 2A, ARIX1 homeodomain protein, Aristaless homeobox protein homolog, Paired-like homeobox 2A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PHOX2A antibody [EPR9071] (AB155084)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2A antibody [EPR9071] (AB155084)

Immunofluorescent analysis of SH-SY-5Y cells, labeling PHOX2A with ab155084 at 1/100 dilution.

Western blot - Anti-PHOX2A antibody [EPR9071] (AB155084)
  • WB

Unknown

Western blot - Anti-PHOX2A antibody [EPR9071] (AB155084)

All lanes:

Western blot - Anti-PHOX2A antibody [EPR9071] (ab155084) at 1/1000 dilution

Lane 1:

SH-SY-5Y cell lysate at 10 µg

Lane 2:

Neuro-2a cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 30 kDa

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Western blot - Anti-PHOX2A antibody [EPR9071] (AB155084)
  • WB

Lab

Western blot - Anti-PHOX2A antibody [EPR9071] (AB155084)

Blocking and diluting buffer and concentration : 5% NFDM /TBST. ab181602 was used as a GAPDH loading control. Exposure time : Lane 1-3 : 180 seconds; Lane 4 : 40 seconds. HEK293 and HeLa are negative controls (PMID : 17505528, PMID : 17344216). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17344216)

All lanes:

Western blot - Anti-PHOX2A antibody [EPR9071] (ab155084) at 1/1000 dilution

Lane 1:

HEK293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

IMR-32 (Human neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-PHOX2A antibody [EPR9071] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR9071

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Primary Antibodies

AB139726

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Immunocytochemistry/ Immunofluorescence - Anti-LMX1A antibody - C-terminal (AB139726)

  • 4
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Primary Antibodies

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Anti-beta III Tubulin antibody [2G10] - Neuronal Marker

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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

  • 5
  • 40
Primary Antibodies

AB183741

Anti-PHOX2B antibody [EPR14423] - C-terminal

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (AB183741)

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Primary Antibodies

AB281588

Anti-MAP2 antibody [RM1010] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

  • 0
  • 0
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Reactivity data

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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出荷温度及び保存条件

製品の状態
Liquid
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存温度
+4°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHOX2A also known as Arix among other names is a transcription factor with an approximate mass of 34 kDa. It plays an essential role in the development of the autonomic nervous system. PHOX2A shows specific expression in neuronal tissues particularly in the noradrenergic and cholinergic neurons. Its function involves the regulation of genes that control neuronal differentiation and development.
Biological function summary

PHOX2A is key in the regulation of noradrenergic phenotype important for the formation and maintenance of the autonomic nervous system. It interacts as part of a transcriptional complex working directly to activate the expression of genes necessary for the synthesis of catecholamines such as dopamine beta-hydroxylase. The protein ensures the proper differentiation of neural crest cells into sympathetic and parasympathetic neurons by influencing its genetic targets.

Pathways

PHOX2A is integral to the development of the autonomic nervous system pathway and the noradrenergic biosynthesis pathway. Much of its function is orchestrated through interactions with other transcription factors like PHOX2B. By regulating gene expression within these pathways PHOX2A contributes to the broader network of neural crest cell development impacting cellular processes that underpin nervous system formation and function.

Mutations in PHOX2A have been linked to congenital central hypoventilation syndrome and Hirschsprung disease. In these conditions PHOX2A's relationships with other proteins notably PHOX2B are significant as these proteins share overlapping roles in neural development. Disruptions in PHOX2A consequently impair pathways and processes critical for autonomic control and gut innervation leading to the aforementioned disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

May be involved in regulating the specificity of expression of the catecholamine biosynthetic genes. Acts as a transcription activator/factor. Could maintain the noradrenergic phenotype.
See full target information PHOX2A
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature 642:143-153 PubMed40205039

2025

Human assembloid model of the ascending neural sensory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Il Kim,Kent Imaizumi,Ovidiu Jurjuț,Kevin W Kelley,Dong Wang,Mayuri Vijay Thete,Zuzana Hudacova,Neal D Amin,Rebecca J Levy,Grégory Scherrer,Sergiu P Pașca

Nature biotechnology : PubMed37974010

2023

Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yunlong Tao,Xueyan Li,Qiping Dong,Linghai Kong,Andrew J Petersen,Yuanwei Yan,Ke Xu,Seth Zima,Yanru Li,Danielle K Schmidt,Melvin Ayala,Sakthikumar Mathivanan,Andre M M Sousa,Qiang Chang,Su-Chun Zhang

Cell reports 33:108425 PubMed33238113

2020

Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans.

Applications

Unspecified application

Species

Unspecified reactive species

R Brian Roome,Farin B Bourojeni,Bishakha Mona,Shima Rastegar-Pouyani,Raphael Blain,Annie Dumouchel,Charleen Salesse,W Scott Thompson,Megan Brookbank,Yorick Gitton,Lino Tessarollo,Martyn Goulding,Jane E Johnson,Marie Kmita,Alain Chédotal,Artur Kania

Journal of cellular and molecular medicine 21:2036-2045 PubMed28272778

2017

The activity of the carbamoyl phosphate synthase 1 promoter in human liver-derived cells is dependent on hepatocyte nuclear factor 3-beta.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanfei Chen,Nanhong Tang,Xiaoqian Wang,Yanling Chen
View all publications
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Abcam product promise

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