Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナント

Anti-Phosphotyrosine 抗体 [RM111] (ab190824)

Submit a review Submit a question References (2)
Western blot - Anti-Phosphotyrosine antibody [RM111] (ab190824)
  • Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)
  • Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (ab190824)
  • ELISA - Anti-Phosphotyrosine antibody [RM111] (ab190824)
  • Anti-Phosphotyrosine antibody [RM111] (ab190824)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM111] to Phosphotyrosine
  • Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IF
  • Reacts with: Species independent

製品の概要

  • 製品名

    Anti-Phosphotyrosine antibody [RM111]
    Phosphotyrosine 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [RM111] to Phosphotyrosine

  • 由来種

    Rabbit

  • 特異性

    This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non phosphorylated tyrosine, phospho-serine, and phospho-threonine

  • アプリケーション

    適用あり: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IFmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to Bovine Serum Albumin (BSA).

  • ポジティブ･コントロール

    • Serum starved, EGF treated A431 cells; serum starved, EGF treated A431 cell lysate; EGF treated, A431 cell lysate.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab190824の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000.
IP
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
1/200 - 1/500.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ChIP
1/200 - 1/1000.
ICC/IF
1/200 - 1/500.
特記事項
WB
1/1000 - 1/5000.
IP
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
1/200 - 1/500.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ChIP
1/200 - 1/1000.
ICC/IF
1/200 - 1/500.

ターゲット情報

  • 関連性

    The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

画像

  • Western blot - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    Western blot - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    All lanes : Anti-Phosphotyrosine antibody [RM111] (ab190824) at 1/5000 dilution

    Lanes 1 & 3 & 5 : Non treated serum-starved A431 cell lysate
    Lanes 2 & 4 & 6 : EGF treated serum-starved A431 cell lysate


    The blot was was subjected twice to increasing exposure time in Lanes 3 and 4, and Lanes 5 and 6.

  • Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)

    Immunoprecipitation of EGF-treated A431 cells pulling down Phosphotyrosine-EGFR by ab190824 at 1/1000 dilution; this was followed by western blot with an anti-EGFR rabbit monoclonal antibody.
    (1) Whole lysate control;

    (2) IP by rabbit IgG control;

    (3) IP by ab190824

  • Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (ab190824)

    Immunocytochemistry of serum-starved A431 cells nontreated (Left) or treated with EGF (Right) labeling Phosphotyrosine-EGFR using ab190824 at 1/500 dilution  (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

  • ELISA - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    ELISA - Anti-Phosphotyrosine antibody [RM111] (ab190824)

    Analysis of ab190824 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. ab190824 only reacted to phosphorylated Tyrosine.

  • Anti-Phosphotyrosine antibody [RM111] (ab190824)
    Anti-Phosphotyrosine antibody [RM111] (ab190824)

参考文献 (2)

ab190824 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab190824 は 2 報の論文で使用されています。

  • Wang Q  et al. Natural variation of ZmLNG1 alters organ shapes in maize. New Phytol 237:471-482 (2023). PubMed: 36266960
  • Xu X  et al. 17ß-Estradiol nongenomically induces vascular endothelial H2S release by promoting phosphorylation of cystathionine ?-lyase. J Biol Chem 294:15577-15592 (2019). PubMed: 31439665

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab190824.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com