AB190824

Anti-Phosphotyrosine 抗体 [RM111]

Anti-Phosphotyrosine antibody [RM111]

Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Phosphotyrosine antibody. Suitable for IP, ChIP, Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Modified Amino Acid samples. Cited in 4 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.

別名を表示する

L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (AB190824)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Immunocytochemistry of serum-starved A431 cells nontreated (Left) or treated with EGF (Right) labeling Phosphotyrosine-EGFR using ab190824 at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Immunoprecipitation of EGF-treated A431 cells pulling down Phosphotyrosine-EGFR by ab190824 at 1/1000 dilution; this was followed by western blot with an anti-EGFR rabbit monoclonal antibody.
(1) Whole lysate control;

(2) IP by rabbit IgG control;

(3) IP by ab190824

All lanes:

Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)

false

Supplier Data

Western blot - Anti-Phosphotyrosine antibody [RM111] (AB190824)

The blot was was subjected twice to increasing exposure time in Lanes 3 and 4, and Lanes 5 and 6.

All lanes:

Western blot - Anti-Phosphotyrosine antibody [RM111] (ab190824) at 1/5000 dilution

Lanes 1, 3 and 5:

Non treated serum-starved A431 cell lysate

Lanes 2, 4 and 6:

EGF treated serum-starved A431 cell lysate

false

ELISA - Anti-Phosphotyrosine antibody [RM111] (AB190824)

ELISA

Supplier Data

ELISA - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Analysis of ab190824 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. ab190824 only reacted to phosphorylated Tyrosine.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

RM111

アイソタイプ

IgG

キャリアフリー

アプリケーション

ICC/IF, ChIP, WB, Flow Cyt, IP, IHC-P, ELISA

applications

特異性

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non phosphorylated tyrosine, phospho-serine, and phospho-threonine

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/1000", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "1/200 - 1/1000", "ChIP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

Purified from an animal origin–free culture supernatant.

バッファー組成

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.

Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Phosphotyrosine

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

The New phytologist 237:471-482 PubMed36266960

2022

Natural variation of ZmLNG1 alters organ shapes in maize.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Wang,Jiongjiong Fan,Jia Cong,Mengjiao Chen,Jie Qiu,Jie Liu,Xiaoyan Zhao,Ruipeng Huang,Hongjun Liu,Xuehui Huang

Experimental and therapeutic medicine 24:480 PubMed35761813

2022

Vitamin E-coated dialyzer alleviates erythrocyte deformability dysfunction in patients with end-stage renal disease undergoing hemodialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Wei Gao,Xia Li

Frontiers in physiology 12:712400 PubMed34504438

2021

Regulates the Proliferation and Migration of Vascular Smooth Muscle Cells in the Restenosis Process Post-PTA in Lower Extremity Arteries.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Zhou,Lixing Qi,Yongquan Gu

The Journal of biological chemistry 294:15577-15592 PubMed31439665

2019

17β-Estradiol nongenomically induces vascular endothelial HS release by promoting phosphorylation of cystathionine γ-lyase.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyan Xu,Qing Yan,Xiaoyun Liu,Ping Li,Xiaosa Li,Yiwen Chen,Tommaso Simoncini,Junxiu Liu,Dongxing Zhu,Xiaodong Fu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphotyrosine 抗体 [RM111]

Anti-Phosphotyrosine antibody [RM111]

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Western blot - Anti-Phosphotyrosine antibody [RM111] (AB190824)

ELISA - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

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Primary Antibodies

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