Anti-Phosphotyrosine 抗体 [IG2] (ab5592)
![ELISA - Anti-Phosphotyrosine antibody [IG2] (ab5592)](https://www.abcam.co.jp/ps/products/5/ab5592/Images/ab5592-467349-anti-phosphotyrosine-antibody-elisa.jpg "ELISA - Anti-Phosphotyrosine antibody [IG2] (ab5592)")
Key features and details
- Mouse monoclonal [IG2] to Phosphotyrosine
- Suitable for: ELISA, ICC/IF, WB, ICC, Flow Cyt, IP
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-Phosphotyrosine antibody [IG2]
Phosphotyrosine 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [IG2] to Phosphotyrosine -
由来種
Mouse -
特異性
ab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides. -
アプリケーション
適用あり: ELISA, ICC/IF, WB, ICC, Flow Cyt, IPmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Phosphotyrosine. O-phospho-L-tyrosine and O-phospho-DL-tyramine.
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ポジティブ･コントロール
- HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
IG2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5592の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use a concentration of 1 µg/ml.
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| ICC/IF |
1/50 - 1/200.
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| WB |
1/100 - 1/1000.
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| ICC |
1/50 - 1/200.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IP |
Use a concentration of 2 µg/ml.
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| 特記事項 |
|---|
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ELISA
Use a concentration of 1 µg/ml. |
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ICC/IF
1/50 - 1/200. |
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WB
1/100 - 1/1000. |
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ICC
1/50 - 1/200. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IP
Use a concentration of 2 µg/ml. |
ターゲット情報
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関連性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
画像
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ELISA - Anti-Phosphotyrosine antibody [IG2] (ab5592)
Direct ELISA of a 96 well plate coated with 100 uL per well of phosphotyrosine, phosphoserine or phosphothreonine at 5 µg/mL, incubated with 100 µL per well of ab5592
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![Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)](https://www.abcam.co.jp/ps/products/5/ab5592/Images/ab5592_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)Immunolocalization of phosphotyrosine in HeLa cells using ab5592.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab5592 は論文での使用が確認できていません。