AB5592

Anti-Phosphotyrosine 抗体 [IG2]

Name: Anti-Phosphotyrosine 抗体 [IG2] (ab5592) | Abcam
Brand: Abcam Limited
SKU: AB5592
Price: 75000 JPY
Availability: InStock

Anti-Phosphotyrosine antibody [IG2]

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(2 Publications)

Mouse Monoclonal Phosphotyrosine antibody. Suitable for ICC, IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Modified Amino Acid samples. Cited in 2 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.

別名を表示する

L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (AB5592)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (AB5592)

Immunolocalization of phosphotyrosine in HeLa cells using ab5592.

ELISA - Anti-Phosphotyrosine antibody [IG2] (AB5592)

ELISA

Supplier Data

ELISA - Anti-Phosphotyrosine antibody [IG2] (AB5592)

Direct ELISA of a 96 well plate coated with 100 uL per well of phosphotyrosine, phosphoserine or phosphothreonine at 5 μg/mL, incubated with 100 μL per well of ab5592

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

IG2

アイソタイプ

IgG1

キャリアフリー

アプリケーション

IP, ELISA, ICC, Flow Cyt, ICC/IF, WB

applications

特異性

ab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "1/50 - 1/200", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "2 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.

Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Phosphotyrosine

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmacology research & perspectives 13:e70167 PubMed40842442

2025

6-Nitrodopamine Release From Mouse Seminal Vesicles Is Dependent on Endothelial Nitric Oxide Synthase (eNOS) Activation.

Applications

Unspecified application

Species

Unspecified reactive species

José Britto-Júnior,Pérola Rafaella Cedano Godoy,Denis Oliveira Lima,Valéria B de Souza,André A Schenka,Fernanda V Mariano,Felipe Fernandes Jacintho,Idam Hermawan,Hiroaki Shimokawa,Masato Tsutsui,Silvana Chiavegatto,Edson Antunes,Gilberto De Nucci

Nature communications 16:5875 PubMed40595687

2025

Arginine regulates the mucoid phenotype of hypervirulent Klebsiella pneumoniae.

Applications

Unspecified application

Species

Unspecified reactive species

Brooke E Ryan,Caitlyn L Holmes,Drew J Stark,Grace E Shepard,Emma G Mills,Saroj Khadka,Daria Van Tyne,Michael A Bachman,Laura A Mike

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphotyrosine 抗体 [IG2]

Anti-Phosphotyrosine antibody [IG2]

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (AB5592)

ELISA - Anti-Phosphotyrosine antibody [IG2] (AB5592)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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Abcam product promise