AB9319

Anti-Phosphotyrosine 抗体

Name: Anti-Phosphotyrosine 抗体 (ab9319) | Abcam
Brand: Abcam Limited
SKU: AB9319
Price: 76000 JPY
Availability: InStock
Rating: 4 (2 reviews)

Anti-Phosphotyrosine antibody

(2 Reviews)

(11 Publications)

Rabbit Polyclonal Phosphotyrosine antibody. Suitable for IP, ELISA, WB, IHC-P, IHC-Fr and reacts with Modified Amino Acid samples. Cited in 11 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.

別名を表示する

L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphotyrosine antibody (AB9319)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphotyrosine antibody (AB9319)

Immunohistochemistry analysis of human breast cancer with ab9319

Supplier Data

Western blot - Anti-Phosphotyrosine antibody (AB9319)

All lanes:

Western blot - Anti-Phosphotyrosine antibody (ab9319) at 0.5 µg/mL

Lane 1:

5 ul of crude protein from of HeLa cell lysate treated with fetal calf serum at 2 mg/mL

Lane 2:

15 ul of crude protein from HeLa cell lysate treated with fetal calf serum at 2 mg/mL

Lane 3:

5 ul of crude protein from HeLa cell lysate at 2 mg/mL

Lane 4:

15 ul of crude protein from HeLa cell lysate at 2 mg/mL

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

アプリケーション

IHC-P, IHC-Fr, ELISA, WB, IP

applications

特異性

Recognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "10 µg/500 µg protein sample.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine.

バッファー組成

pH: 6 - 8 Preservative: 0.01% Sodium azide Constituents: 0.114% Tris buffered saline

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.

Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Phosphotyrosine

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

The European journal of neuroscience 60:6935-6952 PubMed39543939

2024

Amyloid beta-activated alpha-1-syntrophin has ramifications on Rac1 activation, ROS production and neuronal cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Umar Mushtaq,Rais A Ganai,Muzamil Ahmad,Firdous Ahmad Khanday

Inflammatory bowel diseases 27:1804-1812 PubMed34019094

2021

Unique Regulation of Coupled NaCl Absorption by Inducible Nitric Oxide in a Spontaneous SAMP1/YitFc Mouse Model of Chronic Intestinal Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Subha Arthur,Balasubramanian Palaniappan,Sheuli Afroz,Uma Sundaram

Nucleic acids research 48:5777-5787 PubMed32352518

2020

Spatial organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers.

Applications

Unspecified application

Species

Unspecified reactive species

Toon Verheyen,Trixy Fang,Dominik Lindenhofer,Yang Wang,Karen Akopyan,Arne Lindqvist,Björn Högberg,Ana I Teixeira

The American journal of pathology 188:640-655 PubMed29305319

2018

Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Komal Ramani,Maria Lauda Tomasi,Joshua Berlind,Nirmala Mavila,Zhaoli Sun

Scientific reports 6:29246 PubMed27385592

2016

Protease activated receptor 1 (PAR1) enhances Src-mediated tyrosine phosphorylation of NMDA receptor in intracerebral hemorrhage (ICH).

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Zhen Duan,Feng Zhang,Feng-Ying Li,Yi-Fei Luan,Peng Guo,Yi-Hang Li,Yong Liu,Su-Hua Qi

The Journal of biological chemistry 291:11736-50 PubMed27022017

2016

Do Src Kinase and Caveolin Interact Directly with Na,K-ATPase?

Applications

Unspecified application

Species

Unspecified reactive species

Eliyahu Yosef,Adriana Katz,Yoav Peleg,Tevie Mehlman,Steven J D Karlish

Scientific reports 5:13474 PubMed26310847

2015

Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Satoki Nakamura,Tomoaki Kahyo,Hong Tao,Kiyoshi Shibata,Nobuya Kurabe,Hidetaka Yamada,Kazuya Shinmura,Kazunori Ohnishi,Haruhiko Sugimura

Nature methods 11:841-6 PubMed24997862

2014

Spatial control of membrane receptor function using ligand nanocalipers.

Applications

Unspecified application

Species

Unspecified reactive species

Alan Shaw,Vanessa Lundin,Ekaterina Petrova,Ferenc Fördős,Erik Benson,Abdullah Al-Amin,Anna Herland,Andries Blokzijl,Björn Högberg,Ana I Teixeira

The Journal of biological chemistry 287:19367-76 PubMed22505718

2012

Molecular cloning and characterization of first organic matrix protein from sclerites of red coral, Corallium rubrum.

Applications

Species

Unspecified reactive species

Julien Debreuil,Eric Tambutté,Didier Zoccola,Emeline Deleury,Jean-Marie Guigonis,Michel Samson,Denis Allemand,Sylvie Tambutté

The Journal of neuroscience : the official journal of the Society for Neuroscience 30:15521-34 PubMed21084607

2010

Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.

Applications

Species

Unspecified reactive species

Zhihui Zhong,Yaoming Wang,Huang Guo,Abhay Sagare,José A Fernández,Robert D Bell,Theresa M Barrett,John H Griffin,Robert S Freeman,Berislav V Zlokovic

View all publications

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Abcam product promise

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Anti-Phosphotyrosine 抗体

Anti-Phosphotyrosine antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphotyrosine antibody (AB9319)

Western blot - Anti-Phosphotyrosine antibody (AB9319)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

アプリケーション

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (11)

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Abcam product promise