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    products/primary-antibodies/phosphothreonine-proline--phosphoserine-proline-antibody-ab9344.pdf

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Signal Transduction Protein Phosphorylation pSer / pThr
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Anti-Phosphothreonine-Proline / Phosphoserine-Proline 抗体 (ab9344)

  • Datasheet
Submit a review Q&A (6)References (11)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)

    Key features and details

    • Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline
    • Suitable for: ELISA, WB
    • Reacts with: Species independent
    • Isotype: IgG

    こちらの製品もご検討ください

    一次抗体
    Product image
    Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
    タンパク質
    Product image
    Recombinant Human Histone H1 protein (ab198676)
    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    関連製品

    製品の概要

    • 製品名

      Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody
    • 製品の詳細

      Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline
    • 由来種

      Rabbit
    • 特異性

      This antibody specifically reacts to proteins containing phosphothreonine-proline motifs, it also reacts to the phosphoserine-proline motif to a similar degree (pT-P and pS-P motif). The antibody does not react to phosphothreonine, phosphoserine or phosphotyrosine. The antibody will selectively recognize the phosphothreonine-proline motif of MBP, which is the site of phosphorylation by ERK1/ERK2.
    • アプリケーション

      適用あり: ELISA, WBmore details
    • 種交差性

      交差種: Species independent
    • 免疫原

      Chemical/ Small Molecule corresponding to Phosphothreonine-Proline/ Phosphoserine-Proline conjugated to keyhole limpet haemocyanin.

    • ポジティブ・コントロール

      • WB: Phosphorylated MBP
    • 特記事項


      The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 6
      Preservative: 0.02% Sodium azide
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      Immunoaffinity chromatography with phosphothreonine-proline on agarose, then immuno-absorption with threonine-proline-NH2 on agarose.
    • 一次抗体 備考

      The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Protein Phosphorylation
      • pSer / pThr
      • Neuroscience
      • Neurotransmission
      • Intracellular Signaling
      • Regulation

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9344の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA
    Use a concentration of 0.5 µg/ml.
    WB
    Use a concentration of 2 µg/ml.
    特記事項
    ELISA
    Use a concentration of 0.5 µg/ml.
    WB
    Use a concentration of 2 µg/ml.

    画像

    • Western blot - Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)
      Western blot - Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)
      All lanes : Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)

      Lane 1 : Phosphorprotein protein kinase A PKA
      Lane 2 : Myelin Basic Protein (MBP)

    プロトコール

    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (11)

    ab9344 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9344 は 11 報の論文で使用されています。

    • Zhang P  et al. K63-linked ubiquitination of DYRK1A by TRAF2 alleviates Sprouty 2-mediated degradation of EGFR. Cell Death Dis 12:608 (2021). PubMed: 34117217
    • Ryu KJ  et al. p38 Stabilizes Snail by Suppressing DYRK2-Mediated Phosphorylation That Is Required for GSK3ß-ßTrCP-Induced Snail Degradation. Cancer Res 79:4135-4148 (2019). PubMed: 31209060
    • Murthy T  et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
    • Lei T  et al. Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation. Nat Commun 9:1876 (2018). PubMed: 29760377
    • Wang H  et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). PubMed: 28607489
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    Hi,

    Thanks for the information, I am still keen to participate. If you could send me a discount code I can the order the antibody.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2012

    Answer

    xxxxx

    I am very pleased to hear you would like to accept our offer and test ab9344 in IF. The code above will give you £227 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site:

    https://www.abcam.com/Abreviews

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests whatever they may be. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.

    If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here:

    https://www.abcam.com/abtrial

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2012

    Question

    Hi,

    I am still interested in purchasing this antibody and I would require it for both western blotting and for immunofluorescence. As the IF is untested would I qualify for a testing discount?

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2012

    Answer

    Thank you for your reply.

    Kate is currently not in the office but I will try to help with your enquiry as best I can. If you were to test this antibody in IF you would be eligible to take part in the Abtrial testing program.

    The way that this program works it that you would purchase ab9344 as normal, test the antibody in IF and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab9344 off any product in our catalogue. More information on this offer can be found here:

    https://www.abcam.com/abtrial

    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab9344.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Oct 24 2012

    Question

    Hi,

    I emailed you last Friday after meeting you at a symposium at Leicester Uni on fridy. I wondered if you had any more info about how I can try the ab9344 anti-phospho Ser/Pro antibody using the abreviews system?

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 15 2012

    Answer

    Thank you for your message which has been forwarded to the scientific support team.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. I can confirm that ab9344 Phosphothreonine-Proline / Phosphoserine-Proline antibody has been tested in WB and ELISA.

    If the product is to be used in an untested application, you may indeed be eligible for our testing discount program if the antibody has not yet been purchased. Please contact me again prior to the purchase by replying to this email and I will be pleased to provide more information.

    Alternatively, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 15 2012

    Question

    To whom it may concern,   I am interested in buying the Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344). I am doing a kinase assay to detect JNK phosphorylation of various substrates in an ELISA format. Would this antibody work for an application like this?  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2011

    Answer

    Thank you for contacting Abcam. The antibody ab9344 is guaranteed to work in ELISAs and so would most probably work in the application that you intend to use it for, however you would have to work out the exact condition for you experiment, but I think it should be ok. If there is anything else I can help you with, please let me kn

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2011

    Question

    Customer would like to know if we can offer anti proline-glycine-glycine or anti proline-glycine-proline antibodies.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 11 2011

    Answer

    Thank you for contacting us. We have anti glycine or anti- proline antibodies in our catalogue. The catalogue numbers are ab9344, ab9345, ab37067, ab9442 and ab37087. We unfortunately do not have antibodies available specifically raised against proline-glycine-proline short peptide. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html Elastin proteins also have repeating units of proline and glycine residues. So probably monoclonal antibodies against part of Elastin will be helpful e.g. ab97963 and ab51599. Although these do not exactly match however it will help to widen your search. I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

    Read More

    Abcam Scientific Support

    Answered on Aug 11 2011

    Question

    Does the antibody specifically recognise the phosphorylated site of myelin basic protein by p38 MAPK?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 21 2003

    Answer

    It is not tested. If there is no steric hindrance, it should be able to do so.

    Read More

    Abcam Scientific Support

    Answered on Oct 27 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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