Anti-Phosphoserine 抗体 (ab9332)
Key features and details
- Rabbit polyclonal to Phosphoserine
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-Phosphoserine antibody
Phosphoserine 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phosphoserine -
由来種
Rabbit -
特異性
Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin. KLH-phosphoserine conjugate and phosvitin mixture.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.9% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9332の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (7) |
Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
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| 特記事項 |
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WB
Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding. |
ターゲット情報
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関連性
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family. -
別名
- phospho-Ser antibody
- pS antibody
- pSER antibody
画像
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Western blot - Anti-Phosphoserine antibody (ab9332)This image is courtesy of an Abreview submitted by Karin Birkenkamp-DemtroederAll lanes : Anti-Phosphoserine antibody (ab9332) at 3 µg/ml (in PBS for 16 hours)
All lanes : Whole cell lysate of monkey COS7 cells
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution
Blocking Step: 10% Milk for 1 hour at room temperature -
Western blot - Anti-Phosphoserine antibody (ab9332)Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (156)
ab9332 は 156 報の論文で使用されています。
- Saxena K et al. FAT10 is phosphorylated by IKKβ to inhibit the antiviral type-I interferon response. Life Sci Alliance 7:N/A (2024). PubMed: 37940187
- Zhao T et al. NRF1-mediated mitochondrial biogenesis antagonizes innate antiviral immunity. EMBO J 42:e113258 (2023). PubMed: 37409632
- Wang Y et al. Phosphorylation of enteroviral 2Apro at Ser/Thr125 benefits its proteolytic activity and viral pathogenesis. J Med Virol 95:e28400 (2023). PubMed: 36511115
- Jia Y et al. Tripartite motif containing 69 elicits ERK2-dependent EYA4 turnover to impart pancreatic tumorigenesis. J Cancer 14:200-218 (2023). PubMed: 36741265
- Yoon I et al. EPRS1 Controls the TGF-β Signaling Pathway via Interaction with TβRI in Hepatic Stellate Cell. Mol Cell Biol 43:223-240 (2023). PubMed: 37154023