Anti-Phosphoribosyl pyrophosphate amidotransferase 抗体 (ab125864)
Key features and details
- Rabbit polyclonal to Phosphoribosyl pyrophosphate amidotransferase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Phosphoribosyl pyrophosphate amidotransferase antibody
Phosphoribosyl pyrophosphate amidotransferase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phosphoribosyl pyrophosphate amidotransferase -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 283-467 of Human Phosphoribosyl pyrophosphate amidotransferase (NP_002694).
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ポジティブ･コントロール
- WB: HepG2 and HCT116 whole cell lysates. IF: HeLa whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125864の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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組織特異性
Ubiquitously expressed. -
パスウェイ
Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. -
配列類似性
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
Contains 1 glutamine amidotransferase type-2 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 5471 Human
- Omim: 172450 Human
- SwissProt: Q06203 Human
- Unigene: 331420 Human
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別名
- Amidophosphoribosyltransferase [Precursor] antibody
- Amidophosphoribosyltransferase antibody
- ATase antibody
see all
画像
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Western blot - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864)All lanes : Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864)Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling Phosphoribosyl pyrophosphate amidotransferase with ab125864 at 1/500 dilution. Costained with Hoechst 33342 (Bottom panel).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab125864 は 1 報の論文で使用されています。
- Hu X et al. Identification and Validation of Novel Biomarkers for Diagnosis and Prognosis of Hepatocellular Carcinoma. Front Oncol 10:541479 (2020). PubMed: 33102213