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RabMAb

Anti-Phospholipase D1 抗体 [EP1506Y] (ab68150)

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Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)
  • Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)

Key features and details

  • Rabbit monoclonal [EP1506Y] to Phospholipase D1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Phospholipase D1 antibody [EP1506Y]
    Phospholipase D1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1506Y] to Phospholipase D1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, NIH/3T3 and C6 whole cell lysate.

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68150の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
特記事項
WB
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.

  • 組織特異性

    Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.

  • 配列類似性

    Belongs to the phospholipase D family.
    Contains 1 PH domain.
    Contains 2 PLD phosphodiesterase domains.
    Contains 1 PX (phox homology) domain.

  • 細胞内局在

    Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Choline phosphatase 1 antibody
    • hPLD1 antibody
    • Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
    • Phospholipase D1 antibody
    • PLD 1 antibody
    • PLD1 antibody
    • PLD1_HUMAN antibody
    see all

画像

  • Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)
    Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)
    All lanes : Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) (RIPA) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) (RIPA) whole cell lysate
    Lane 3 : C6 (Rat glial tumor glial cell) (RIPA) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 120 kDa
    Observed band size: 120 kDa


    Exposure time: 60 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBS

  • Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)
    Western blot - Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)
    Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 120 kDa
    Observed band size: 120 kDa

参考文献 (3)

ab68150 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab68150 は 3 報の論文で使用されています。

  • Stricker HM  et al. The phospholipase D inhibitor FIPI potently blocks EGF-induced calcium signaling in human breast cancer cells. Cell Commun Signal 19:43 (2021). PubMed: 33832505
  • Wang Z  et al. Binding of PLD2-Generated Phosphatidic Acid to KIF5B Promotes MT1-MMP Surface Trafficking and Lung Metastasis of Mouse Breast Cancer Cells. Dev Cell 43:186-197.e7 (2017). PubMed: 29033361
  • Sulzmaier FJ  et al. PEA-15 potentiates H-Ras-mediated epithelial cell transformation through phospholipase D. Oncogene 31:3547-60 (2012). PubMed: 22105357

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