AB76155

Anti-Phospholipase C gamma 1/PLC-gamma-1 抗体 [EP1898-7Y]

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y]

20ul selling size
RabMAb
Recombinant
KO Validated
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(14 Publications)

Rabbit Recombinant Monoclonal Phospholipase C gamma 1/PLC-gamma-1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 14 publications.

別名を表示する

PLC1, PLCG1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-II, PLC-gamma-1

2 Images

Lab

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (AB76155)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Phospholipase C gamma 1/PLC-gamma-1 knockout HAP1 cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lane 4 : Mouse brain tissue lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab76155 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab76155 was shown to specifically react with Phospholipase C gamma 1/PLC-gamma-1 when Phospholipase C gamma 1/PLC-gamma-1 knockout samples were used. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab76155 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)

Predicted band size: 148 kDa

false

Unknown

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (AB76155)

All lanes:

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155) at 1/10000 dilution

All lanes:

Jurkat cell lysates at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 148 kDa

Observed band size: 149 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1898-7Y

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C gamma 1 (PLC-gamma-1) also known as PLCG1 or PLC-gamma plays an important role in cellular signaling. This enzyme has a molecular mass of approximately 150 kDa. It is found in multiple cell types but is highly expressed in the brain and immune cells. PLC-gamma-1 is involved in the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) to generate inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) leading to downstream signaling events that control various cellular processes.

Biological function summary

PLC-gamma-1 is integral in signal transduction and is an important player in the immune response. The protein does not function as part of a large multi-protein complex but interacts transiently with other signaling molecules. It acts downstream of growth factor receptors and immunoreceptors by mediating calcium release and activation of protein kinase C influencing cell proliferation migration and differentiation. Its expression pattern suggests a critical role in the nervous and immune systems affecting learning memory and immune defense.

Pathways

PLC-gamma-1 is an important component in both the phospholipase C and PLC-gamma pathways. These pathways include important interactions with proteins such as the receptor tyrosine kinases and Src family kinases. In these pathways PLC-gamma-1 modulates signals that guide cellular responses to external stimuli affecting processes like growth signaling and T-cell receptor activation which is vital in adaptive immunity.

PLC-gamma-1 has significant connections to cancer and immune deficiencies. Abnormal PLC-gamma-1 activity is observed in certain cancers such as breast cancer where it influences tumor growth and metastasis. Its dysregulation in immune cells leads to disorders like autoimmune diseases by impacting proteins such as LAT and SLP-76 which are important in T-cell signaling. Understanding PLC-gamma-1’s regulatory mechanisms helps in developing targeted therapies for these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Plays a role in actin reorganization and cell migration (PubMed : 17229814). Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis (By similarity).

See full target information PLCG1

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e31860 PubMed38841509

2024

Qi Lang formula relieves constipation via targeting SCF/c-kit signaling pathway: An integrated study of network pharmacology and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiacheng Li,Yugang Fu,Yanping Wang,Yiyuan Zheng,Kehui Zhang,Yong Li

Journal of ginseng research 47:89-96 PubMed36644379

2023

Polysaccharides from promote intestinal epithelial cell migration through affecting the Ca related regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Huibin Zhu,Jianhong Cao,Xinyi Liang,Meng Luo,Anrong Wang,Ling Hu,Ruliu Li

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:155-172 PubMed36384680

2022

Selective TLR4 Antagonism Prevents and Reverses Morphine-Induced Persistent Postoperative Cognitive Dysfunction, Dysregulation of Synaptic Elements, and Impaired BDNF Signaling in Aged Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie M Muscat,Nicholas P Deems,Michael J Butler,Emmanuel A Scaria,Menaz N Bettes,Sean P Cleary,Ross H Bockbrader,Steven F Maier,Ruth M Barrientos

Theranostics 12:3316-3328 PubMed35547746

2022

NAD-boosting molecules suppress mast cell degranulation and anaphylactic responses in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Woo Kim,Ga-Hee Ryoo,Hyun-Young Jang,So-Young Rah,Dong Hyun Lee,Do-Kyun Kim,Eun Ju Bae,Byung-Hyun Park

Evidence-based complementary and alternative medicine : eCAM 2021:6076293 PubMed34925531

2021

Paeoniflorin Improved Constipation in the Loperamide-Induced Rat Model via TGR5/TRPA1 Signaling-Mediated 5-Hydroxytryptamine Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhan,Yong Wen,Liang-Liang Zhang,Xu-Long Shen,Xiao-Hui Chen,Xiao-Hai Wu,Xue-Gui Tang

Cell proliferation 54:e13081 PubMed34121240

2021

A novel multikinase inhibitor SKLB-YTH-60 ameliorates inflammation and fibrosis in bleomycin-induced lung fibrosis mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyao Liu,Xiuli Wu,Cailing Gan,Liqun Wang,Guan Wang,Lin Yue,Zhihao Liu,Wei Wei,Xingping Su,Qianyu Zhang,Zui Tan,Yuqin Yao,Liang Ouyang,Luoting Yu,Tinghong Ye

Nutrition & metabolism 18:56 PubMed34082784

2021

Aerobic exercise promotes the functions of brown adipose tissue in obese mice via a mechanism involving COX2 in the VEGF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyu Fu,Rongxin Zhu,Jie Jia,Yang Hu,Chengjun Wu,Pawel Cieszczyk,Hans-Christer Holmberg,Lijing Gong

Theranostics 10:11013-11025 PubMed33042267

2020

Toll-like receptor 5 deficiency diminishes doxorubicin-induced acute cardiotoxicity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Guo Ma,Chun-Yan Kong,Hai-Ming Wu,Peng Song,Xin Zhang,Yu-Pei Yuan,Wei Deng,Qi-Zhu Tang

Brain and behavior 10:e01592 PubMed32157827

2020

Activation of brain-derived neurotrophic factor signaling in the basal forebrain reverses acute sleep deprivation-induced fear memory impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ma,Hao Zhang,Zhi-Peng Xu,Yan Lu,Qiang Fu,Wei Wang,Guan-Hua Li,Ying-Ying Wang,Yi-Tian Yang,Wei-Dong Mi

Bioscience reports 40: PubMed31922200

2020

Couplet medicines of leech and centipede granules improve erectile dysfunction via inactivation of the CaSR/PLC/PKC signaling in streptozotocin-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xiong Ma,Bin Wang,Cai Fei Ding,Hai Song Li,Xue Juan Jiang,Chen Ye Wang,Jia Yu,Wang Qiang Chen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phospholipase C gamma 1/PLC-gamma-1 抗体 [EP1898-7Y]

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y]

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (AB76155)

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (AB76155)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (14)

Abcam product promise