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リコンビナントRabMAb

Anti-Phospholipase A2 activator protein (PLAP) 抗体 [Y102] (ab32354)

Reviews (1)Q&A (2)References (4)
Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)
  • Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y102] to Phospholipase A2 activator protein (PLAP)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102]
    Phospholipase A2 activator protein (PLAP) 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y102] to Phospholipase A2 activator protein (PLAP)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Phospholipase A2 activator protein (PLAP) aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • MCF-7 and K562 cell lysates and human lung metastatic cancer tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    Y102

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32354の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Involved in the maintenance of ubiquitin levels.

  • 配列類似性

    Belongs to the WD repeat PLAP family.
    Contains 6 ARM repeats.
    Contains 1 PFU domain.
    Contains 1 PUL domain.
    Contains 7 WD repeats.

  • ドメイン

    The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
    The PFU domain mediates interaction with ubiquitin.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ11281 antibody
      • FLJ12699 antibody
      • Glycerophosphatase antibody
      • OTTHUMP00000045176 antibody
      • Phospholipase A 2 activating protein antibody
      • Phospholipase A-2-activating protein antibody
      • Phospholipase A2 activating protein antibody
      • PLA2P antibody
      • Plaa antibody
      • PLAP antibody
      • PLAP_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)
      Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)
      All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : K562 cell lysate

      Predicted band size: 80 kDa
      Observed band size: 81 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)

      Immunohistochemical analysis of paraffin-embedded human lung metastatic cancer using ab32354 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)
      Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (4)

    ab32354 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32354 は 4 報の論文で使用されています。

    • Hülsmann J  et al. AP-SWATH Reveals Direct Involvement of VCP/p97 in Integrated Stress Response Signaling Through Facilitating CReP/PPP1R15B Degradation. Mol Cell Proteomics 17:1295-1307 (2018). PubMed: 29599191
    • Lin Q  et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468
    • Zhang F  et al. Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA). Cell Signal 20:844-61 (2008). PubMed: 18291623
    • Alexandru G  et al. UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1alpha turnover. Cell 134:804-16 (2008). PubMed: 18775313

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (fibroblasts culture)
    Loading amount
    30 µg
    Specification
    fibroblasts culture
    Gel Running Conditions
    Reduced Denaturing (10% SDS-page gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Yishay Shoval

    Verified customer

    投稿 Jan 04 2012

    Question

    thank you for your offer of a replacement for our antibody. Since the anti-Plaa ab32354 did not work well, and no other anti-Plaa antibody is available, we have decided to move from the endogenous to an exogenously expressed protein. We have obtained a 6-His tagged Plaa expression plasmid, and are therefore seeking an anti-6-His tag antibody. As a replacement, we would ask for ab18184 (https://www.abcam.com/6X-His-tag-antibody-HIS-H8-ab18184.html). the anti-Plaa antibody was purchased 3 months ago.

    Read More
    Answer

    Thank you for your reply. I would be happy to provide a replacement vial of ab18184. To do so I will just need to access the original order on our system. Please provide the Abcam order reference number or an internal purchase order number so I can process your request. If you have purchased the antibody through one of our distributors, please request the order information from them. I look forward to hearing from you. Please let me know if you have any other questions

    Read More

    Question

    Please send sequence and/or the exact position of the immunogenic peptide in the protein.

    Read More
    Answer

    Thank you for your enquiry. The exact location of the peptide is proprietary information but it is between aa 70 - 90. I hope this helps. Please do not hesitate to contact us if you have any more questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com