Anti-Phospholamban (phospho S16 + T17) 抗体 (ab62170)
Key features and details
- Rabbit polyclonal to Phospholamban (phospho S16 + T17)
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Phospholamban (phospho S16 + T17) antibody
Phospholamban 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phospholamban (phospho S16 + T17) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human Phospholamban (phospho S16 + T17).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62170の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. -
組織特異性
Heart muscle (at protein level). -
関連疾患
Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18 -
配列類似性
Belongs to the phospholamban family. -
翻訳後修飾
Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. -
細胞内局在
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). - Information by UniProt
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参照データベース
- Entrez Gene: 18821 Mouse
- Entrez Gene: 64672 Rat
- SwissProt: P61014 Mouse
- SwissProt: P61016 Rat
- Unigene: 34145 Mouse
- Unigene: 9740 Rat
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別名
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Phospholamban (phospho S16 + T17) antibody (ab62170)Immunofluorescence analysis of HuvEc cells, using Phospholamban (phospho S16 + T17) antibody (ab62170) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab62170 は 5 報の論文で使用されています。
- Gonçalves LS et al. Histidine dipeptides are key regulators of excitation-contraction coupling in cardiac muscle: Evidence from a novel CARNS1 knockout rat model. Redox Biol 44:102016 (2021). PubMed: 34038814
- Qin R et al. Exercise training reduces ventricular arrhythmias through restoring calcium handling and sympathetic tone in myocardial infarction mice. Physiol Rep 7:e13972 (2019). PubMed: 30806037
- Chen J et al. Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells. Exp Ther Med 18:4631-4636 (2019). PubMed: 31798700
- Kim GE et al. LKB1 deletion causes early changes in atrial channel expression and electrophysiology prior to atrial fibrillation. Cardiovasc Res 108:197-208 (2015). PubMed: 26378152
- Huang Y et al. Sarcoplasmic phospholamban protein is involved in the mechanisms of postresuscitation myocardial dysfunction and the cardioprotective effect of nitrite during resuscitation. PLoS One 8:e82552 (2013). WB ; Rat . PubMed: 24386101