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Anti-Phospholamban 抗体 (ab86930)

Submit a review Q&A (2)References (7)
Western blot - Anti-Phospholamban antibody (ab86930)
  • Western blot - Anti-Phospholamban antibody (ab86930)

Key features and details

  • Rabbit polyclonal to Phospholamban
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Phospholamban antibody [EPR21897] (ab219626)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Phospholamban antibody
    Phospholamban 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Phospholamban

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human Phospholamban aa 2-16 (N terminal).
    Sequence:

    EKVQYLTRSAIRRAS


    Database link: P26678
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Rat heart tissue lysate. CEM, MCF-7, HT1080, and HeLa cell lysates

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab86930の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 6 kDa.
特記事項
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 6 kDa.

ターゲット情報

  • 機能

    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.

  • 組織特異性

    Heart muscle (at protein level).

  • 関連疾患

    Cardiomyopathy, dilated 1P
    Cardiomyopathy, familial hypertrophic 18

  • 配列類似性

    Belongs to the phospholamban family.

  • 翻訳後修飾

    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.

  • 細胞内局在

    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cardiac phospholamban antibody
    • CMD1P antibody
    • CMH18 antibody
    • PLB antibody
    • Pln antibody
    • PPLA_HUMAN antibody
    see all

画像

  • Western blot - Anti-Phospholamban antibody (ab86930)
    Western blot - Anti-Phospholamban antibody (ab86930)
    All lanes : Anti-Phospholamban antibody (ab86930) at 0.5 µg/ml

    Lanes 1-2 : Rat Heart Tissue Lysate at 50 µg
    Lane 3 : CEM Whole Cell Lysate at 40 µg
    Lane 4 : MCF-7 Whole Cell Lysate at 40 µg
    Lane 5 : HT1080 Whole Cell Lysate at 40 µg
    Lane 6 : HeLa Whole Cell Lysate at 40 µg

    Predicted band size: 6 kDa

  • Western blot - Anti-Phospholamban antibody (ab86930)
    Western blot - Anti-Phospholamban antibody (ab86930)
    All lanes : Anti-Phospholamban antibody (ab86930) at 1 µg/ml

    All lanes : Rat heart tissue lysate

    Predicted band size: 6 kDa
    Observed band size: 11 kDa why is the actual band size different from the predicted?

参考文献 (7)

ab86930 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab86930 は 7 報の論文で使用されています。

  • Miranda-Silva D  et al. Disturbed cardiac mitochondrial and cytosolic calcium handling in a metabolic risk-related rat model of heart failure with preserved ejection fraction. Acta Physiol (Oxf) 228:e13378 (2020). PubMed: 31520455
  • Silveira AC  et al. Exercise Training Restores Cardiac MicroRNA-1 and MicroRNA-29c to Nonpathological Levels in Obese Rats. Oxid Med Cell Longev 2017:1549014 (2017). PubMed: 29138674
  • Yavari A  et al. Mammalian ?2 AMPK regulates intrinsic heart rate. Nat Commun 8:1258 (2017). PubMed: 29097735
  • Seldin MM  et al. A systems genetics approach identifies Trp53inp2 as a link between cardiomyocyte glucose utilization and hypertrophic response. Am J Physiol Heart Circ Physiol 312:H728-H741 (2017). PubMed: 28235788
  • Najafi A  et al. Selective phosphorylation of PKA targets after ß-adrenergic receptor stimulation impairs myofilament function in Mybpc3-targeted HCM mouse model. Cardiovasc Res 110:200-14 (2016). PubMed: 26825555
  • Melo SF  et al. Exercise training restores the cardiac microRNA-1 and -214 levels regulating Ca2+ handling after myocardial infarction. BMC Cardiovasc Disord 15:166 (2015). WB ; Rat . PubMed: 26646371
  • Locatelli J  et al. Swimming training promotes cardiac remodeling and alters the expression of mRNA and protein levels involved in calcium handling in hypertensive rats. Life Sci 117:67-74 (2014). PubMed: 25283082

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Hi:
I am sorry to bother you.
I want to do western blot to detect the expression of phospholamban and phospho-phospholamban on guinea pig bladder smoothe muscle. I want to use the antibodies as following: ab85146(phospholamban) and ab15000(phospho-phospholamban)
Meanwhile, I have some questions and need your help.
1. I found two antibodies(ab85146 & ab86930) for phospholamban and one antibody(ab15000) for phospho-phospholamban. But I didn't find they can react with guinea pig in cross reactivity of their datasheet.
So, I want know whether the three antibodies can react with guinea pig of not.
2. I want to know the difference betweeb ab85146 and ab86930 and which one is more suitable for guinea pig.

Read More
Answer

Thank you for contacting us. We have not tested these antibodies in guinea pig and therefore cannot guarantee they will work, however there is high homology between the immunogen and the guinea pig sequence for all three. Normally we predictlikely reactivity if there is 85% homology of the immunogen with the species, and for ab15000 there is 91%, ab85146 is 90% and ab86930 is 93%. The two phospholambin antibodies ab85146 and ab86930 are very similar. They are both rabbit polyclonal purified antibodies and have similar likelihood of reactiving with guinea pig. They do have difference concentrations and volumes (ab85146 is provided at 1ug/ml and 100 ul; ab86930 is 0.5 ug/ml and 200 ul) and have different immunogens. Either would be a good choice.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hi: I want to detect the protein expression of Phospholamban and Phospholamban(phospho S16) on Guinea pig bladder smoothe muscle, in the presence or absence of drugs by western blot.So I want to know as following about ab86930, ab85146 and ab15000 as following: 1. What is the difference between ab86930 and ab85146? Which is more suitable for guinea pig? 2. Are there other antibodies more suitable for Phospholamban and Phospholamban(phospho S16) on guinea pig? 3.Which lysate solution is best for Phospholamban and Phospholamban(phospho S16)? 4.Which protease and phosphatase inhibitors are best for the two protein? 5.Would you like give the protocal for the phosphorylation protein,such as phospho S16?

Read More
Answer

Thank you for contacting Abcam.

I would be very happy to help answer your questions:

1 - What is the difference between ab86930 and ab85146? Which is more suitable for guinea pig?

- Both antibodies target different areas of the Phospholamban protein. For a lot of targets, Abcam offers multiply antibodies, that either target different domains, or are more suited to different applications, depending on the customers’ needs.

I cannot find the guinea pig sequence for Phospholamban, therefore I cannot make a recommendation as to whether either of these antibodies would work for that species. If you could please supply the guinea pig sequence, I would be happy to do the alignment and see if there is a chance that either will work for you.

2 - Are there other antibodies more suitable for Phospholamban and Phospholamban(phospho S16) on guinea pig?

- Again, without the guinea pig sequence, I cannot confidently answer this question.

3 - Which lysate solution is best for Phospholamban and Phospholamban(phospho S16)?

- From the information available on the webpages, it looks as though mouse, rat, human and bovine cardiac tissue either treated with or without PKA would be suitable lysates.

4 - Which protease and phosphatase inhibitors are best for the two protein?

- This would be determined on your exact experiment at hand, below is a link to some protease inhibitors, however if you require phospahatse inhibitors then, we unfortunately do not carry those in our catalogue:

https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html



5. Would you like give the protocol for the phosphorylation protein,such as phospho S16?

- For ab15000 was treated with PKA to induce phosphorylation.

Please let me know if there is anything else I can help you with, or if you would like to me to perform the alignment between the guinea pig sequence and the immunogen sequences for these antibodies.

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